Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Rapid and robust isolation of microglia and vascular cells from brain subregions for integrative single-cell analyses

ABSTRACT: Rapid and robust isolation of microglia and vascular cells from brain subregions for integrative single-cell analyses

PROVIDER: PRJNA1125356 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR29454529.fastq.gz	Fastqsanger.gz
	SRR29454530.fastq.gz	Fastqsanger.gz
	SRR29454531.fastq.gz	Fastqsanger.gz
	SRR29454532.fastq.gz	Fastqsanger.gz
	SRR29454533.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 10

Similar Datasets

Rapid and robust isolation of microglia and vascular cells from brain subregions for integrative single-cell analyses

Project description:Cell isolation protocols from brain tissue constitute prolonged ex vivo processing durations, rendering them suboptimal for transcriptomic studies. Εspecially, yielding sufficient quantities of microglia or vascular cells requires the use of large tissue volumes and the addition of an enrichment step. In this study, we developed a simple, rapid, and reproducible cell isolation method for generation of single-cell suspensions from micro-dissected brain regions, providing high numbers of microglia and vascular cells, without requiring an additional encrihment step. Cells isolated using this method are suitable for molecular profiling studies using 10× Genomics Chromium single-cell RNA sequencing with high reproducibility. Our method is valuable for longitudinal unbiased molecular profiling of microglia and vascular cells within different brain regions, spanning multiple time points across physiological development or disease progression.

2024-09-30 | GSE270158 | GEO

Assessment of RNA Isolation Kit Biases Toward Preferential Detection of exRNA Cargo Types

Project description:An integrative analysis of human biofluid data in the exRNA Atlas revealed the existence of distinct extracellular RNA cargo types. To determine whether different RNA isolation kits biased detection of certain exRNA cargo types, an integrative analysis was performed using pooled plasma and serum samples, where 10 different RNA isolation kits were applied.

2018-12-15 | GSE123865 | GEO

Rapid, high efficiency isolation of pancreatic ß-cells

Project description:The ability to isolate pure pancreatic ß-cells would greatly aid multiple areas of diabetes research. We developed an exendin-4-like neopeptide conjugate for the rapid purification and isolation of functional pancreatic ß-cells. By targeting the glucagon-like peptide-1 receptor, ß-cells were isolated within an hour and were >99% pure. These studies were confirmed by immunostaining, confocal microscopy and microarray analysis on isolated cells. Gene expression profiling studies of the cytofluorometrically sorted ß-cells provided new insights into the genetic programs at play of different ages and stages during type-1 diabetes development. The described isolation method should have broad applicability to the ß-cell field.

2015-09-04 | GSE64508 | GEO

MicorRNA expression in klotho-deficient and wild-type mouse brain subregions

Project description:The expression profiles of miRNAs in klotho-deficient and wild-type mouse brain subregions were examined. The data indicated that little or no marked difference in the miRNA expression was detected between them. Twelve samples prevared from 4- or 5-week-old klotho-deficient and wild-type mouse brain tissues (cerebrum, cerebellum and hippocampus) were examined.

2011-06-08 | E-GEOD-29092 | biostudies-arrayexpress

MicorRNA expression in klotho-deficient and wild-type mouse brain subregions

2011-06-08 | GSE29092 | GEO

A robust and efficient microvascular isolation method for multimodal characterization of the mouse brain vasculature

Project description:A robust and efficient microvascular isolation method for multimodal characterization of the mouse brain vasculature

| PRJNA884993 | ENA

Distinct reward processing by subregions of the nucleus accumbens

Project description:The nucleus accumbens (NAc) plays an important role in motivation and reward processing. Recent studies suggest that different NAc subnuclei differentially contribute to reward-related behaviors. However, how reward is encoded in individual NAc neurons remains unclear. Using in vivo single-cell resolution calcium imaging, we discovered diverse patterns of reward encoding in the medial and lateral shell subdivision of the NAc (NAcMed and NAcLat, respectively). Reward consumption increases NAcLat activity but decreases NAcMed activity, albeit with high variability among neurons. The heterogeneity in reward encoding could be attributed to differences in their synaptic inputs and transcriptional profiles. Specific optogenetic activation of Nts-positive neurons in the NAcLat promotes positive reinforcement, while activation of Cartpt-positive neurons in the NAcMed induces behaviour aversion. Collectively, our study reveals organizational and transcriptional differences in NAc subregions, and provides a framework for future dissection of NAc subregions in physiological and pathological conditions.

2023-01-14 | GSE222274 | GEO

Rapid, high efficiency isolation of pancreatic ß-cells

2015-09-04 | E-GEOD-64508 | biostudies-arrayexpress

Integrative Epigenomic and Transcriptomic Analysis Reveals Robust Metabolic Switching During Intermittent Fasting in the Brain

Project description:Integrative Epigenomic and Transcriptomic Analysis Reveals Robust Metabolic Switching During Intermittent Fasting in the Brain

| PRJNA560714 | ENA

The subiculum is a patchwork of discrete subregions

Project description:The subiculum is a patchwork of discrete subregions

| PRJNA449925 | ENA