Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

IMPACT OF NEAR-CONTINUOUS LOW DOSE-RATE NEUTRON IRRADIATION ON PREGNANCY OUTCOMES IN MICE

ABSTRACT: The effects of galactic cosmic radiation on reproductive physiology remain largely unknown. We determined the impact of near-continuous low-dose-rate Californium-252 neutron irradiation (1 mGy/day) as a space-relevant analog on litter size and number of resorptions at embryonic day (E) 12.5 (n=19 radiated dams, n=20 controls) and litter size, number of resorptions, fetal growth, and placental signaling and transcriptome (RNA sequencing) at E18.5 (n=21 radiated dams, n=20 controls) in pregnant mice. A significantly increased early resorption rate and decreased placental weight was observed in irradiated mice. There were no statistically significant differences in litter size, fetal weight, length, or malformation rate between the groups. Near-continuous radiation had no significant effects on mechanistic target of rapamycin (mTOR), endoplasmic reticulum stress or inflammatory signaling, rate of double-stranded DNA breaks, and had minimal effects on gene expression in the placenta. These data suggest that near-continuous, low-level galactic cosmic radiation has a limited impact on pregnancy outcomes.

ORGANISM(S): Mus musculus

PROVIDER: GSE271237 | GEO | 2024/07/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Galactic cosmic radiation induces stable epigenome alterations relevant to human lung cancer

Project description:Galactic cosmic radiation induces stable epigenome alterations relevant to human lung cancer

| PRJNA422769 | ENA

Approaches for Surveying Cosmic Radiation Damage in Large Populations of Arabidopsis thaliana Seeds- an Antarctic Example

Project description:The Cosmic Ray Exposure Sequencing Science (CRESS) payload system is a proof of concept experiment to assess the genomic impact of space radiation on seeds. CRESS was designed as a secondary payload for the December 2016 high-altitude, high-latitude, and long-duration balloon flight carrying the Boron And Carbon Cosmic Rays in the Upper Stratosphere (BACCUS) experimental hardware. Investigation of the biological effects of Galactic Cosmic Radiation (GCR), particularly those of ions with High-Z and Energy (HZE), is of interest due to the genomic damage this type of radiation inflicts. The biological effects of upper-stratospheric mixed radiation above Antarctica (ANT) were sampled using Arabidopsis thaliana seeds and were compared to those resulting from a controlled simulation of GCR at Brookhaven National Laboratory (BNL) and to laboratory control seed. The payload developed for Antarctica exposure was broadly designed to 1U CubeSat specifications (10cmx10cmx10cm, ≤1.33kg), maintained 1 atm internal pressure, and carried an internal cargo of four seed trays (about 580,000 seeds) and twelve CR-39 Solid-State Nuclear Track Detectors (SSNTDs). The irradiated seeds were recovered, sterilized and grown on Petri plates for phenotypic screening. BNL and ANT M0 seeds showed significantly reduced germination rates and elevated somatic mutation rates when compared to non-irradiated controls, with the BNL mutation rate also being significantly higher than that of ANT. Genomic DNA from mutants of interest was evaluated with whole-genome sequencing using PacBio SMRT technology. Sequence data revealed the presence of an array of genome structural variants in the genomes of M0 and M1 mutant plants.

2018-12-20 | GSE121111 | GEO

P300-mediated adaptive conversion of glioma stem cells to vascular-like cells in response to therapeutic stress promotes tumor recurrence [RNA-seq_sorted GEC GPC]

Project description:Purpose: The goal of the study is to determine the transcriptional changes associated with non-radiated cells, radiated cells, and sorted non-radiated, and radiation-induced endothelial and pericyte-like cells to establish the identity of the vascular-like cells

2022-09-09 | GSE207729 | GEO

Proteomic and phosphoproteomic characterization of cardiovascular dysfunction arising from exposure to space radiation

Project description:Mice were exposed to 150 cGy Galactic Cosmic Ray (GCR) versus sham, and hearts and plasma were harvested at 8 months post radiation. Hearts were homogenized in 5% SDS and 250 ugs protein was digested with trypsin using an S-trap followed by TMT labeling, high-pH reversed-phase fractionation and phosphopeptide enrichement. 12 fractions (unenriched and phospho-enriched) were analyzed by nanoLC-MS/MS using an MS2 method. 15 uL plasma was processed using sodium-deoxycholate assisted trypsin digestion followed by microflow LC-MS/MS using data-independent acquisition.

2023-07-10 | MSV000092395 | MassIVE

Non-radiated and Radiated lymphocytes from breast cancer patients treated with high-dose hyperfractioned radiotherapy

Project description:The aim of this study was to assess the relationship between gene expression profiles of ex vivo 2 Gy radiated lymphocytes and the development of acute and late toxicity due to hyperfractionated dose-escalation radiation therapy schedule in patientes with advanced breast cancer. The gene expression profiles of ex vivo non-radiated lymphocytes were also examined. Keywords: Toxicity grade analysis.

2009-06-05 | GSE15341 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Differentially Expressed Genes in Radiated and Non-Radiated femurs

Project description:Mechanisms underlying radiation induced bone loss seen following clinical radiotherapy are largely unkown. In order to identify mechanisms underlying focal radiation induced bone loss, differentially expressed genes were assessed in radiated bones as compared to the contralateral leg from the same animal.

2021-07-15 | GSE180076 | GEO

Fractionated Proton Irradiation does not Impair Hippocampal Dependent Short Term or Spatial Memory in Female Mice

Project description:The environment outside the Earth’s protective magnetosphere is a much more threatening and complex space environment. The dominant causes for radiation exposure, solar particle events and galactic cosmic rays, contain high-energy protons. In space, astronauts need healthy and highly functioning cognitive abilities, of which the hippocampus plays a key role. Therefore, understanding the effects of 1H exposure on hippocampal-dependent cognition is vital for de-veloping mitigative strategies and protective countermeasures for future missions. To investi-gate these effects, we subjected 6-month-old female CD1 mice to 0.75 Gy fractionated 1H (250 MeV) whole-body irradiation at the NASA Space Radiation Laboratory. The cognitive perfor-mance of the mice was tested 3 months after irradiation using Y-maze and morris water maze tests. Both sham-irradiated and 1H-irradiated mice significantly preferred exploration of the novel arm compared to the familiar and start arms, indicating intact spatial and short-term memory. Both groups statistically spent more time in the target quadrant, indicating spatial memory retention. There were no significant differences in neurogenic and gliogenic cell counts after irradiation. In addition, proteomic analysis revealed no significant upregulation or down-regulation of proteins related to behavior, neurological disease, or neural morphology. Our data suggests 1H exposure does not impair hippocampal-dependent spatial or short-term memory in female mice.

2022-10-14 | PXD035933 | Pride

Non-radiated and Radiated lymphocytes from breast cancer patients treated with high-dose hyperfractioned radiotherapy

2010-05-18 | E-GEOD-15341 | biostudies-arrayexpress

mRNA and lncRNA expression analysis of irradiated and nonirradiated human HaCaT cells

Project description:This study aims to compare mRNA expression between radiated and non-radiated human keratinocyte HaCaT cells by microarray analysis. Human keratinocyte HaCaT cells were divided into two groups, each group has three repeats. The cells were irradiated with a single dose of 0 or 20Gy of X-ray irradiation. 48 hours post radiation, cells from 0 or 20 Gy groups were collected and subjected to microarray analysis. mRNA and lncRNA profilings of each group were analyzed by microarray.

2018-07-14 | GSE117094 | GEO

P300-mediated adaptive conversion of glioma stem cells to vascular-like cells in response to therapeutic stress promotes tumor recurrence [scRNA-seq in vivo]

Project description:Purpose: The goal of the study is to determine the phenotypic and functional states of glioma cells induce in response to a standard glioblastoma therapy, ionizing radiation. Methods: We performed single-cell RNA-sequencing on non-radiated and radiated tumor cells isolated from patient-derived xenografts. Tumor-bearing mice were exposed to a single dose of radiation ( 8 Gy) and cells were isolated and FACS sorted to obtain cell suspensions for assessing gene expression. Single cell suspensions and cDNA library preparation was done using 10x Genomics Chromium single cell reagent kit v3 according to manufacturer's protocol. cDNA samples were sequenced on 1 lane of NovaSeq 6000 S2 flowcell. Reads were mapped to HumanGRCh38 genome using Cell Ranger v.3.0.2. Seurat package v3.1.1 was used for integrated analysis by Canonical correlation analysis method with 75 dimensions . PCA was used for dimensional reduction, and Cell clustering was performed using shared nearest neighbor method. Each cell cluster was annotated by a combination of the following methods 1) canonical marker expression 2) Gene Set Enrichment Analysis (GSEA) of cluster specific markers determined by Find Markers function in Seurat and 3) Co-expression modules obtained by Louvain community detection clustering method. Trajectory analysis was done with monocle 3 (version 0.2.1.3) by converting Seurat object to monocle. Results: We identified 14 clusters in non-radiated tumors and 15 clusters in radiated tumors.

2022-09-09 | GSE207726 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data