Genomics

Dataset Information

0

The Cytokines IL-21 and GM-CSF have Opposing Regulatory Roles in the Apoptosis of Conventional Dendritic Cells


ABSTRACT: Interleukin-21 (IL-21) has broad actions on T- and B-cells, but its actions in innate immunity are poorly understood. Here we show that IL-21 induced apoptosis of conventional dendritic cells (cDCs) via STAT3 and Bim, and this was inhibited by granulocyte-macrophage colony-stimulating factor (GM-CSF). ChIP-Seq analysis revealed genome-wide binding competition between GM-CSF-induced STAT5 and IL-21-induced STAT3. Expression of IL-21 in vivo decreased cDC numbers, and this was prevented by GM-CSF. Moreover, repetitive α-galactosylceramide injection of mice induced IL-21 but decreased GM-CSF production by natural killer T (NKT) cells, correlating with decreased cDC numbers. Furthermore, adoptive-transfer of wild-type CD4+ T cells caused more severe colitis with increased DCs and interferon (IFN)-γ-producing CD4+ T cells in Il21r-/-Rag2-/- mice (which lack T cells and have IL-21-unresponsive DCs) than in Rag2-/- mice. Thus, IL-21 and GM-CSF exhibit cross-regulatory actions on gene regulation and apoptosis, regulating cDC numbers and thereby the magnitude of the immune response.

ORGANISM(S): Mus musculus

PROVIDER: GSE27161 | GEO | 2013/02/14

SECONDARY ACCESSION(S): PRJNA137403

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2013-02-14 | E-GEOD-27161 | biostudies-arrayexpress
2023-02-23 | GSE210935 | GEO
2015-12-12 | GSE75938 | GEO
2015-12-12 | E-GEOD-75938 | biostudies-arrayexpress
2015-06-26 | E-GEOD-54120 | biostudies-arrayexpress
2013-05-06 | E-GEOD-46270 | biostudies-arrayexpress
2013-05-06 | GSE46270 | GEO
2015-12-12 | E-GEOD-75937 | biostudies-arrayexpress
2015-12-12 | GSE75937 | GEO
2015-12-11 | E-GEOD-71837 | biostudies-arrayexpress