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CUT&RUN sequencing of wild-type and ZNF217-knockdown KOPN-8 cells

ABSTRACT: Here, we elucidate the oncogenic role of ZNF217 in B-ALL and reveal that ZNF217 interacts with the CoREST complex to mediate histone modifications such as H3K4me1, H3K4me2, and H3K27ac. This interaction partially contributes to ZNF217’s oncogenic function in B-ALL. To identify genes directly bound by ZNF217, we conducted high-throughput CUT&RUN sequencing using an anti-ZNF217 antibody in both wild-type and ZNF217-knockdown KOPN-8 cells. Additionally, to distinguish between CoREST-dependent and CoREST-independent targets, we performed CUT&RUN sequencing in the same cell models using an antibody against LSD1, a core component of the CoREST complex that interacts with ZNF217, as well as antibodies against H3K4me1, H3K4me2, and H3K27ac.

ORGANISM(S): Homo sapiens

PROVIDER: GSE272397 | GEO | 2025/02/16

REPOSITORIES: GEO

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