Transcriptomics

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Cell types activated in mouse muscle by TLR7/8 stimulation


ABSTRACT: ScRNA-seq analysis of 11,450 cells from mouse muscle tissue sections following R848 injection identified 19 cell clusters, including four fibroblast, two endothelial cell, two macrophage, and two T cell subpopulations. To identify which cell populations were activated downstream of TLR7/8, interferon gene signature (IFN-GS) and NF-κB-gene signature (NF-κB-GS) scores were calculated and heat maps generated. There was a noticeable IFN response in most cell populations, with the greatest IFN-GS induction in neutrophils, endothelial cells and macrophages, and some response in T cells. However, IFN induction was not observed in any muscle cell type after R848 injection. The NF-κB-GS was expressed under basal conditions in many cell populations and was induced by R848 most in macrophages and neutrophils, with some increase also seen in endothelial cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE272898 | GEO | 2024/09/02

REPOSITORIES: GEO

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