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Escherichia coli growing under antimicrobial gallium nitrate stress reveals new processes of tolerance and toxicity

ABSTRACT: Gallium nitrate has been explored as an FDA-approved alternative to treat antibiotic-resistant bacterial strains. Questions remain as it relates to the full-systems response elicited to counteract its toxic effects. We explored the transcriptomic response profile of Escherichia coli K12 BW25113 when challenged to grow planktonically for 10 hours in the presence of a sublethal concentration of gallium nitrate. Our results show that several biological processes are dysregulated to cope with the presence of gallium nitrate, including iron metabolism, oxidative and nitrosative stress responses, and sulfur metabolism. Additionally, enriched biological systems in the gallium condition include cysteine biosynthesis, anaerobic respiration, toxin-antitoxin interactions, and DNA repair. Altogether, this work provides a valuable snapshot of how our indicator strain adapts to this metal-induced stress. This is a significant step in understanding how bacteria can adjust their physiology to coexist with sublethal concentrations of metal-based antimicrobials.

ORGANISM(S): Escherichia coli

PROVIDER: GSE273773 | GEO | 2025/01/08

REPOSITORIES: GEO

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