Dataset Information

Distinct Secretomes and Proteomes of Vibrio cholerae isolates indued by Sublethal Concentrations of Heavy Metals Cd2+, Ni2+, Pb2+, and Zn2+ Stresses

ABSTRACT: Vibrio cholerae is a Gram-negative bacterium that can cause pandemic cholera in humans. Meanwhile, the aquaculture environment heavy metal pollution situation is serious. In this study, the growths of the V. cholerae isolates recovered from commonly consumed fish were measured for the first time in sublethal concentrations of heavy metals.

ORGANISM(S): Vibrio Cholerae H1 Vibrio Cholerae

SUBMITTER: Beiyu Zhang

PROVIDER: PXD046079 | iProX | Thu Oct 12 00:00:00 BST 2023

REPOSITORIES: iProX

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Publications

Comparative secretomic and proteomic analysis reveal multiple defensive strategies developed by Vibrio cholerae against the heavy metal (Cd2+, Ni2+, Pb2+, and Zn2+) stresses.

Zhang Beiyu B Xu Jingjing J Sun Meng M Yu Pan P Ma Yuming Y Xie Lu L Chen Lanming L

Frontiers in microbiology 20231026

Vibrio cholerae is a common waterborne pathogen that can cause pandemic cholera in humans. The bacterium with heavy metal-tolerant phenotypes is frequently isolated from aquatic products, however, its tolerance mechanisms remain unclear. In this study, we investigated for the first time the response of such V. cholerae isolates (n = 3) toward the heavy metal (Cd2+, Ni2+, Pb2+, and Zn2+) stresses by comparative secretomic and proteo ...[more]

PMID: 37954246

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Project description:Background: The high number of heavy metal resistance genes in the soil bacterium Cupriavidus metallidurans CH34 makes it an interesting model organism to study microbial responses to heavy metals. Results: In this study the transcriptional response of this bacterium was measured after challenging it to a wide range of sub-lethal concentrations of various essential or toxic metals. Considering the global transcriptional responses for each challenge as well as by identifying the overlap in upregulated genes between different metal responses, the sixteen metals could be clustered in three different groups. Additionally, next to the assessment of the transcriptional response of already known metal resistance genes, new metal response gene clusters were identified. The majority of the metal response loci showed similar expression profiles when cells were exposed to different metals, suggesting complex cross-talk at transcriptional level between the different metal responses. The highly redundant nature of these metal resistant regions – illustrated by the large number of paralogous genes – combined with the phylogenetic distribution of these metal response regions within evolutionary related and other metal resistant bacteria, provides important insights on the recent evolution of this naturally soil dwelling bacterium towards a highly metal-resistant strain found in harsh and anthropogenic environments. Conclusions: The metal-resistant soil bacterium Cupriavidus metallidurans CH34 displays myriads of gene expression patterns when exposed to a wide range of heavy metals at non-lethal concentrations. The interplay between the different gene expression clusters points towards a complex cross-regulated regulatory network governing heavy metal resistance in C. metallidurans CH34. Keywords: Cupriavidus metallidurans CH34, transcriptional regulation, heavy metal resistance Two-condition experiments. Comparing samples after induction with heavy metals versus non-induced samples. Biological duplicate or triplicate. Each array contains 3 or 4 technical replicates.

Dataset Information

Distinct Secretomes and Proteomes of Vibrio cholerae isolates indued by Sublethal Concentrations of Heavy Metals Cd2+, Ni2+, Pb2+, and Zn2+ Stresses

Publications

Comparative secretomic and proteomic analysis reveal multiple defensive strategies developed by <i>Vibrio cholerae</i> against the heavy metal (Cd<sup>2+</sup>, Ni<sup>2+</sup>, Pb<sup>2+</sup>, and Zn<sup>2+</sup>) stresses.

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