Transcriptome sequencing of sheep hypothalamus tissue reveals the regulatory role of lncRNA in the mechanism of pubertal estrus initiation
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ABSTRACT: Improving the fertility of sheep is an important goal in sheep breeding as it greatly increases the productivity. Dolang sheep is a typical representative breed of lamb in Xinjiang and is the main local sheep breed and meat source in the region. To explore the genes associated with the initiation of puberty in Dolang sheep, the hypothalamic tissues of Dolang sheep prepubertal, pubertal, and postpubertal periods were collected for RNA-seq analysis on the Illumina platform.
Project description:We applied RNA sequencing analysis to hypothalamuses from prepubertal and pubertal rats. The significant differential expression transcripts were screened out. In order to validate the RNA-seq data, the expression patterns in the samples of lncRNAs and mRNA genes were confirmed by qRT-PCR. This research provides an expression profile of lncRNAs to further research on puberty.
Project description:We applied RNA sequencing analysis to 6 hypothalamuses from pubertal and prepubertal goats. The significant differential expression transcripts were screened out. In order to validate the RNA-seq data, the expression patterns in the samples of lncRNAs and mRNA genes were confirmed by qRT-PCR. This research provides an expression profile of lncRNAs to further research on puberty.
Project description:Transcriptional profiling of Sertoli cells of Monkey origin comparing infant Sertoli cells with pubertal Sertoli cells, isolated from Monkeys in which puberty was induced by extrinsic GnRH administration. Both Infant and pubertal cells were treated with FSH and Testosterone in vitro.
Project description:Transcriptional profiling of Sertoli cells of Monkey origin comparing infant Sertoli cells with pubertal Sertoli cells, isolated from Monkeys in which puberty was induced by extrinsic GnRH administration. Both Infant and pubertal cells were treated with FSH and Testosterone in vitro.
Project description:Puberty marks the end of childhood and achieve sexual maturation and fertility. The role of hypothalamic proteins in regulating puberty onset is unclear. We performed a comprehensive differential proteomics and phosphoproteomics analysis in prepubertal and pubertal goats to determine the roles of hypothalamic proteins and phosphoproteins during the onset of puberty.
Project description:The COPENHAGEN Puberty Study is a combined cross sectional and longitudinal population based cohort study of healthy Danish children and adolescents. The clinical evaluations were performed by trained physicians and included pubertal staging of breast development according to Tanner´s classification evaluated by palpation. As a measure of pubertal onset a testicular volume of 4 ml or a breast tanner stage of B2 or more was used for boys and girls, respectively. The mean age between two examinations where this threshold was reached was used as their age of pubertal onset. Pre- and post-pubertal samples from 20 girls and 31 boys, in total 102 samples, were normalised using a Subset quantile Within-Array Normalization (SWAN) procedure and probes containing SNPs in the CpG or extension sites were removed.
Project description:Purpose: The purpose of this study was to examine whether different developmental competence of the embryos derived from prepubertal and pubertal animals is affected by the heterogeneity of their transcriptomic profiles. Moreover, the expression of molecular markers associated with mitochondrial function, expressed by the genes involved in OXPHOS, might be applied as a predictable marker for embryonic developmental capability.
Project description:During the 1918 influenza pandemic, children experienced substantially lower mortality than adults, a striking but poorly understood finding. Whether this was due to enhanced resistance (reduced virus load) or better tolerance (reduced impact of infection) has not been defined. We found that prepubertal mice infected with H1N1 influenza virus also showed greater survival than infected pubertal mice, despite similar virus loads. To explore mechanisms for the survival differences, we compared gene expression profiles in lung tissue from mice of both age groups during the course of influenza pneumonia.
Project description:During the 1918 influenza pandemic, children experienced substantially lower mortality than adults, a striking but poorly understood finding. Whether this was due to enhanced resistance (reduced virus load) or better tolerance (reduced impact of infection) has not been defined. We found that prepubertal mice infected with H1N1 influenza virus also showed greater survival than infected pubertal mice, despite similar virus loads. To explore mechanisms for the survival differences, we compared gene expression profiles in lung tissue from mice of both age groups during the course of influenza pneumonia.