Cell subset difference of human sun-exposed and un-exposed Skin：preliminary single cell sequencing and biological analysis
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE275491 | GEO | 2024/08/28
REPOSITORIES: GEO
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Project description:Photoaging is cumulative damage that is caused by chronic, repeated solar radiation exposure to skin. Its molecular mechanisms are poorly understood at the level of global gene expression.This study set out to uncover genes and functional modules involved in photoaging at the level of transcription, with the use of skin samples from Chinese women.Using the Illumina microarray platform, we compared the genome-wide expression profiles of 21 pairs of sun-exposed pre-auricular and sun-protected post-auricular skin samples from northern Chinese women.In total, 1,621 significantly regulated genes were identified from skin due to photoaging by microarray analysis. These genes were subjected to functional enrichment analyses with both the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation databases. As revealed by the functional analyses, the up-regulated functional modules in sun-exposed pre-auricular skin were related to various cellular activities in regulation of the skin homeostasis (e.g., the KEGG pathways TGF-beta signaling pathway and ECM-receptor interaction ), whereas the down-regulated functional modules were mostly metabolic-related. Additionally, five selected genes (HOXA5, LEPR, CLDN5, LAMC3, and CGA) identified as differentially-expressed were further confirmed on their expression by quantitative real-time PCR (Q-RT-PCR). Our findings suggest that damage from skin homeostasis and down-regulation of skin metabolism may play important roles in the process of photoaging.
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Project description:Cell subset difference of human sun-exposed and un-exposed Skin：preliminary single cell sequencing and biological analysis
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Project description:The UV radiation induced epigenetic changes play an important role in photoaging, but experimental evidence of histone modification to support this perspective has been scarce. Analyse the histone H3 acetylation pattern in sun-exposed and non-exposed skin by ChIP-chip. 227 genes displayed significant histone H3 hyperacetylation and 81 genes displayed significant histone H3 hypoacetylation in sun-exposed skins.
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