Transcriptomics

Dataset Information

0

Transcriptomic analysis of gene expression pathways impacted by metaxin-2 (MTX-2) deficiency in a C. elegans mutant (mtx-2 KO, (gk444)) vs Wild-type worms (N2 Bristol)


ABSTRACT: MandibuloAcral Dysplasia associated to MTX2 gene (MADaM) is a recently described progeroid syndrome (accelerated aging disease) whose clinical manifestations include skin abnormalities, growth retardation, and cardiovascular diseases. We previously proposed that mtx-2-deficient C. elegans could be used as a model for MADaM and to support this, we present here our comprehensive phenotypic characterization of these worms using atomic force microscopy (AFM), transcriptomic, and oxygen consumption rate analyses. AFM analysis showed that young mtx-2-less worms had a significantly rougher, less elastic cuticle which becomes significantly rougher and less elastic as they age, and abnormal mitochondrial morphology. mtx-2 C. elegans displayed delayed development, decreased pharyngeal pumping, significantly reduced mitochondrial respiratory capacities, and transcriptomics analysis identified perturbations in the aging, TOR, and WNT-signaling pathways. The phenotypic characteristics of mtx-2 worms shown here are analogous to many of the human clinical presentations of MADaM and we believe this validates their use as a model which will allow us to uncover the molecular details of the disease and develop new therapeutics and treatments.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE276882 | GEO | 2024/09/13

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-01-11 | GSE250064 | GEO
2022-09-20 | GSE213588 | GEO
2008-12-06 | GSE9815 | GEO
2014-01-29 | GSE35455 | GEO
2023-06-14 | GSE228475 | GEO
2015-01-24 | E-GEOD-56426 | biostudies-arrayexpress
2015-01-24 | GSE56426 | GEO
| PRJNA360431 | ENA
2017-01-14 | GSE93620 | GEO
2014-01-29 | E-GEOD-35455 | biostudies-arrayexpress