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Genome binding/occupancy profiling of ALKBH5 by high throughput sequencing [CUT&RUN]

ABSTRACT: Alkylation repair homolog protein 5 (ALKBH5) binds to RNA and mediates RNA m6A demethylation. However, whether ALKBH5 binds to DNA is largely unknown. In this study, our CUT&RUN data show that ALKBH5 can bind to DNA in Hepa1-6 cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE277052 | GEO | 2025/02/27

REPOSITORIES: GEO

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Genome binding/occupancy profiling of ALKBH5 by high throughput sequencing [Cut&Tag]

Project description:Alkylation repair homolog protein 5 (ALKBH5) binds to RNA and mediates RNA m6A demethylation. However, whether ALKBH5 binds to DNA is largely unknown. In this study, our CUT&Tag assay identified 12333 peaks in ALKBH5-overexpressing Hepa1-6 cells, indicating that ALKBH5 can bind to DNA.

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Essential role of ALKBH5-mediated RNA demethylation modification in bile acid-induced gastric intestinal metaplasia

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The interacted proteins of ALKBH5 and FTO identified by IP-mass

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