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Molecular profiles, sources and lineage restrictions of stem cells in an annelid regeneration model

ABSTRACT: Regeneration of missing body parts can be observed in diverse animal phyla, but it remains unclear to which extent these capacities rely on shared or divergent principles. Research into this question requires detailed knowledge about the involved molecular and cellular principles in suitable reference models. By combining single-cell RNA sequencing and mosaic transgenesis in the marine annelid Platynereis dumerilii, we map cellular profiles and lineage restrictions during posterior regeneration. Our data reveal cell-type specific injury responses, re-expression of positional identity factors, and the re-emergence of stem cell signatures in multiple cell populations. Epidermis and mesodermal coelomic tissue produce distinct putative posterior stem cells (PSCs) in the emerging blastema. A novel mosaic transgenesis strategy reveals both developmental compartments and lineage restrictions during regenerative growth. Our work supports the notion that posterior regeneration involves dedifferentiation, and reveals molecular and mechanistic parallels between annelid and vertebrate regeneration.

ORGANISM(S): Platynereis dumerilii

PROVIDER: GSE277281 | GEO | 2024/09/21

REPOSITORIES: GEO

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Annelid adult cell type diversity and their pluripotent cellular origins

Project description:We used scRNA-seq to profile 75,218 single cell transcriptomes of Pristina leidyi, a highly regenerative and asexually-reproducing freshwater annelid. We characterized all major adult cell types, their transcription factors and gene networks, and uncovered an abundant population of putative stem cells with a pluripotent signature.

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Annelid methylomes reveal ancestral developmental and ageing-associated epigenetic erosion across Bilateria

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Resolving transcriptional states and predicting lineages in the annelid Capitella teleta using single-cell RNAseq

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