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ASCL1 phospho-regulation during lineage restrictions of embryonic stem cells (ATAC-Seq)

ABSTRACT: ASCL1 phospho-regulation during lineage restrictions of embryonic stem cells (ATAC-Seq)

PROVIDER: PRJNA1111942 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29044611_1.fastq.gz	Fastqsanger.gz
	SRR29044611_2.fastq.gz	Fastqsanger.gz
	SRR29044612_1.fastq.gz	Fastqsanger.gz
	SRR29044612_2.fastq.gz	Fastqsanger.gz
	SRR29044613_1.fastq.gz	Fastqsanger.gz

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ASCL1 phospho-regulation during lineage restrictions of embryonic stem cells (RNA-Seq)

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ASCL1 phospho-regulation during lineage restrictions of embryonic stem cells (ATAC-Seq)

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ASCL1 phospho-regulation during lineage restrictions of embryonic stem cells (RNA-Seq)

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Project description:Regeneration of missing body parts can be observed in diverse animal phyla, but it remains unclear to which extent these capacities rely on shared or divergent principles. Research into this question requires detailed knowledge about the involved molecular and cellular principles in suitable reference models. By combining single-cell RNA sequencing and mosaic transgenesis in the marine annelid Platynereis dumerilii, we map cellular profiles and lineage restrictions during posterior regeneration. Our data reveal cell-type specific injury responses, re-expression of positional identity factors, and the re-emergence of stem cell signatures in multiple cell populations. Epidermis and mesodermal coelomic tissue produce distinct putative posterior stem cells (PSCs) in the emerging blastema. A novel mosaic transgenesis strategy reveals both developmental compartments and lineage restrictions during regenerative growth. Our work supports the notion that posterior regeneration involves dedifferentiation, and reveals molecular and mechanistic parallels between annelid and vertebrate regeneration.

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FOXA2 and ASCL1 Cooperate in Driving Neuronal Lineage Commitment in Prostate Cancer [RNA-seq]

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Project description:ChIP-Seq analysis performed on 5 ASCL1(+) cell lines and 2 ASCL1 (-) cell lines in order to understand the transcriptome of ASCL1 as it pertains to high-grade neuroendocrine lung cancers 5 ASCL1(+) lung cancer cell lines and 2 ASCL1(-) lung cancer cell lines were compared using ChIP-Seq analysis

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