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Transcriptomic profile of TLR7-induced DN2 B cell development with and without IL-4 Interference in vitro using B cells isolated from SLE patients.

ABSTRACT: The effects of IL-4 on B cell development under an in vitro condition to polarize B cells into the double-negative 2 lineage were investigated. Using single-cell RNA sequencing (scRNA-seq), we analyzed the transcriptomic profiles of B cells from systemic lupus erythematosus (SLE) patients treated with or without IL-4 at 4 hrs and 18 hrs following the stimulation. Our results revealed that IL-4 pre-treatment led to a decrease in the expression of interferon response genes and DN2 signature genes, while promoting the emergence of a B cell population characterized by DN4 signature genes. This has become evident at the 18 hr time point.

ORGANISM(S): Homo sapiens

PROVIDER: GSE278546 | GEO | 2025/04/13

REPOSITORIES: GEO

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