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Transcriptomic Profile of TLR7-Induced B Cell Development in Lupus Mouse Spleen In Vivo, with and without IL-4 Interference

ABSTRACT: We investigated how IL-4 affects age-related B cell development in the context of TLR7 activation. Using single-cell RNA sequencing (scRNA-seq), we analyzed the transcriptomic profiles of autoimmune mice administered R848 with or without IL-4 treatment in vivo. Our results reveal that the differentiation of B cells into DN2 or DN4 stages is established early during the naive phase. IL-4 appears to redirect B cell development towards the transitional stage 2 (T2) and follicular (FO) pathways, while simultaneously inhibiting the formation of T3, marginal zone precursor, and marginal zone B cells in response to R848 stimulation. The transcriptomic data indicate that IL-4 promotes a T-dependent developmental program and suppresses a TLR/BCR-dependent program. IL-4 favors the differentiation of B cells into the precursor of DN4 lineage over the DN2 lineage.

ORGANISM(S): Mus musculus

PROVIDER: GSE278488 | GEO | 2025/04/13

REPOSITORIES: GEO

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