Neutrophils physically interact with tumor cells to form a signaling niche promoting breast cancer aggressiveness

ABSTRACT: Tissue remodeling and cell plasticity in the mammary gland are activated by multi-lineage communications. However, the dynamic signaling promoting breast cancer remains unclear. Here, by RNA-sequencing of single cells and physically interacting cells (PIC-seq) along mammary gland development and carcinogenesis, we uncovered that neutrophils appear transiently during early development and re-emerge in physical interaction with tumor cells in advanced carcinoma. Neutrophil heterogeneity analysis characterized transcriptional states linked to age and cancer stage. Integrating ligand-receptor and PIC-seq analyses with various functional experiments unveiled a physical and secreted pro-tumorigenic signaling niche. This approach revealed that neutrophils are recruited by ductal macrophages and physically interact with tumor cells, increasing tumor cell proliferative and invasive properties, as well as endothelial proliferation and angiogenesis. The molecular program upregulated in neutrophil-PICs correlates with lower survival in advanced breast cancer patients. Our interaction-driven perspective highlights potential molecular targets and biomarkers for breast cancer treatment.

ORGANISM(S): Mus musculus

PROVIDER: GSE278570 | GEO | 2024/12/22

REPOSITORIES: GEO