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Rice A20/AN1 zinc-finger containing stress-associated proteins (SAP1/11) and a receptor-like cytoplasmic kinase (OsRLCK253) interact via A20 zinc-finger and confer abiotic stress tolerance in transgenic Arabidopsis plants

ABSTRACT: The inbuilt mechanisms of plant survival have been exploited for improving tolerance to abiotic stresses. We have investigated the subcellular interactions of rice stress-associated proteins (SAPs) using yeast two-hybrid and FRET approaches and found that A20 domain mediates the interaction of OsSAP1 with self, its close homolog OsSAP11, and a rice receptor-like cytoplasmic kinase, OsRLCK253. Such interactions between OsSAP1/11 and with OsRLCK253 occur at nuclear membrane, plasma membrane and in nucleus. Functionally, both OsSAP11 and OsRLCK253 could improve the water-deficit and salt stress tolerance in transgenic Arabidopsis plants via a signaling pathway affecting the expression of several common endogenous genes. The yield in transgenic plants is also protected indicating the agronomic relevance of OsSAP11 and OsRLCK253 in conferring abiotic stress tolerance.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE27861 | GEO | 2011/05/01

SECONDARY ACCESSION(S): PRJNA137885

REPOSITORIES: GEO

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Rice A20/AN1 zinc-finger containing stress-associated proteins (SAP1/11) and a receptor-like cytoplasmic kinase (OsRLCK253) interact via A20 zinc-finger and confer abiotic stress tolerance in transgenic Arabidopsis plants

Project description:The inbuilt mechanisms of plant survival have been exploited for improving tolerance to abiotic stresses. We have investigated the subcellular interactions of rice stress-associated proteins (SAPs) using yeast two-hybrid and FRET approaches and found that A20 domain mediates the interaction of OsSAP1 with self, its close homolog OsSAP11, and a rice receptor-like cytoplasmic kinase, OsRLCK253. Such interactions between OsSAP1/11 and with OsRLCK253 occur at nuclear membrane, plasma membrane and in nucleus. Functionally, both OsSAP11 and OsRLCK253 could improve the water-deficit and salt stress tolerance in transgenic Arabidopsis plants via a signaling pathway affecting the expression of several common endogenous genes. The yield in transgenic plants is also protected indicating the agronomic relevance of OsSAP11 and OsRLCK253 in conferring abiotic stress tolerance. Arabidopsis transgenic plants expressing rice genes OsSAP11 and OsRLCK253 were compared with untransformed plants at seedling level

2011-05-01 | E-GEOD-27861 | biostudies-arrayexpress

Expression data from leaves of rice plants overexpressing the OsCPK4 gene and control plants(empty vector) in normal conditions

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The wheat C2H2 zinc finger protein TaZFP13D modulates the expression of chloroplast-related genes, improves growth and drought stress tolerance.

Project description:Background Maintaining global food security in the context of climate changes is an important challenge in the next century. Improving abiotic stress tolerance of major crops, like wheat, can contribute to this goal. Therefore, new genes improving tolerance to abiotic stresses, like drought, are needed to support breeding programs aimed at producing more adapted cultivars. Recently, we screened five wheat Zinc Finger Proteins (TaZFPs) and identified TaZFP13D as a new gene improving water-stress tolerance. However, a more detailed characterization of this gene is required to better evaluate its potential in drought tolerance and to decipher the underlying molecular basis. Results We used the Barley Stripe Mosaic Virus to up- or down-regulate TaZFP13D expression in wheat. Overexpression of TaZFP13D under well-watered conditions enhances growth as indicated by improved biomass. Exposing plants to a severe drought stress revealed that TaZFP13D strongly increases survival rate and stress recovery. In addition, TaZFP13D reduces drought-induced oxidative damages, at least in part by improving key antioxidant enzymes activity. Conversely, down-regulation of TaZFP13D decreases drought tolerance and protection against drought-induced oxidative damages. RNA-seq-based transcriptome analysis showed that many genes regulated by TaZFP13D were previously shown to improve drought tolerance, while many others are related to the photosynthetic electron transfer chain and are proposed to improve photosynthesis efficiency and chloroplast protection against ROS damages. Conclusion This study highlights the important role of TaZFP13D in wheat drought tolerance, contributes to unravel the complex regulation governed by TaZFPs, and provides a useful marker to select more drought tolerant wheat cultivars.

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The Submergence Tolerance Regulator Sub1A Mediates Stress-responsive Expression of AP2/ERF Transcription Factors

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Transcriptome analysis to uncover potential roles of a barley ASR gene in stress tolerance

Project description:Whole genome microarray expression profiling was used to identify potential downstream targets and understand cellular processes regulated by a plant ASR protein, particularly HvASR5 from barley, involved in abiotic stress tolerance in cereals. When expressed ectopically, HvASR5 is able to improve growth performance of rice plants under stress conditions. For this experiment, leaf samples from transgenic rice lines overexpressing HvASR5 were compared to wild-type plants. HvASR5 can up-regulate the expression of a distinct set of genes associated with stress responses, metabolic processes, as well as reproduction and development.

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The Submergence Tolerance Regulator Sub1A Mediates Stress-responsive Expression of AP2/ERF Transcription Factors

2010-03-08 | E-GEOD-17704 | biostudies-arrayexpress

Transcription profiling of rice indica after glycinebetaine-induced water-stress tolerance in codA-expressing transgenics

Project description:Glycinebetaine-induced water-stress tolerance in codA-expressing transgenic indica rice is associated with up-regulation of several stress responsive genes. Rice (Oryza sativa L.), a non-accumulator of glycinebetaine (GB), is highly susceptible to abiotic stress. Transgenic rice with chloroplast-targeted choline oxidase encoded by the codA gene from Arthrobacter globiformis has been evaluated for inheritance of transgene up to R5 generation and water-stress tolerance. During seedling, vegetative and reproductive stages, transgenic plants could maintain higher activity of photosystem II and they show better physiological performance, e.g. enhanced detoxification of reactive oxygen species compared to wild-type plants under water-stress. Survival rate and agronomic performance of transgenic plants is also better than wild-type following prolonged water-stress. Choline oxidase converts choline into glycinebetaine and H2O2 in a single step. It is possible that H2O2 /GB might activate stress response pathways and prepare transgenic plants to mitigate stress. To check this possibility, microarray-based transcriptome analysis of transgenic rice has been done. It unraveled altered expression of many genes involved in stress responses, signal transduction, gene regulation, hormone signaling and cellular metabolism. Overall, 165 genes show more than 2 folds up-regulation at P value <0.01 in transgenic rice. Out of these, at least 50 genes are known to be involved in plant stress response. Exogenous application of H2O2 or GB to wild-type plants also induces such genes. Our data show that metabolic engineering for GB is a promising strategy for introducing stress tolerance in crop plants and which could be imparted, in part, by H2O2- and/or GB-induced stress response genes. Experiment Overall Design: Rice (Oryza sativa L.), transgenic plants expressing codA gene from Arthrobacter globiformis were compared with untransformed plants at seedling level

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