Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HalALMT1 mediates malate efflux in the cortex of mature cluster rootlets of Hakea laurina, occurring naturally in phosphorus-poor soil

ABSTRACT: Hakea laurina, a woody plant in Proteaceae family, naturally occurs in severely phosphorus (P)-impoverished environments in southwest Australia. It develops distinctive “cluster roots” that exhibit a high capacity for carboxylate exudation and acid phosphatase activity, contributing to P acquisition. However, the molecular mechanisms underlying these physiological functions remain poorly understood. We explored the cluster root transcriptome using de novo RNA-Seq, which detected homologous transcripts of an aluminum (Al)-activated malate transporter (ALMT), designated HalALMT1, and two multidrug and toxic compound extrusion (MATE) proteins specifically expressed in mature cluster roots. Deferentially-expressed genes highlighted multiple carboxylate-related processes at maturity. Electrophysiological assays and overexpressed transgenic experiments confirmed HalALMT1’s ability to release malate and that its activity is enhanced by exposure to Al3+. Notably, HalALMT1 was specifically expressed in the mature cortex cells of cluster rootlets, which do not develop a suberized exodermis. Furthermore, acid phosphatase activity was pronounced throughout the rootlets, whereas it was only present in the epidermis and stele of non-cluster roots, revealing its extensive production in the rootlets. These results suggest that malate release and acid phosphatase production in the dominant cortex cells in cluster rootlets increase the amount exuded. This study sheds light on an outstanding P-acquisition strategy of Proteaceae.

ORGANISM(S): Hakea laurina

PROVIDER: GSE279190 | GEO | 2024/12/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

A naturally unique variation in Al-activated malate transporter 9 determines fruit malate in cultivated tomato

Project description:Purpose: Understanding the mechanism of malate accumulation in plants is beneficial for both plant nutrient and human health. However, little is known regarding regulation of malate accumulation in horticulture plants such as tomato. The goals of this study are to investigate potential regulation network of Al-Activated Malate Transporter 9 (ALMT9) which is the major locus responsible for the variation in fruit malate concentration observed in cultivared tomato. Methods: The transcriptomes of fruit at ripening stage from CK and SlALMT9 overexpressing line1 were evaluated using single-ended sequencing of Illumina HiSeq 2500. Raw sequences were filtered and the resulting sets of clean reads were used for the following analysis by tophat and edgeR software. Results: Among 1,887 differentially expressed genes, some structural genes involved in sugar and organic acid metabolism were differently expressed in SlALMT9 overexpressing line, which partially coincide with changes of sugar and organic acid concentration. PR genes was also altered in the OX lines, in agreement with previous study that tomato fruit malate is involved in pathogen infection. Three members of malate transporters, MATE (multidrug and toxin efflux), were up-regulated (SlMATE5 and 6) or down-regulated (SlMATE9) . Besides, three sugar transport proteins and four vacuole membrane or plasma membrane proteins were up-regulated in OX line Conclusions: Using RNA-seq analysis, 1,887 differentially expressed genes was revealed. Among them, several genes related to sugar and organic acid metabolism and transport, pathogen resistances were identified. This transcriptome study provides insight into the regulatory mechanism of fruit malate accumulation and the effect of the SlALMT9 to primary metabolism.

2018-02-16 | GSE89772 | GEO

L.hongkongensis transcriptomes grown in media supplemented with or without malate

Project description:To better understand the malate-mediated metabolism and related regulatory profiles of L. hongkongensis, we compared the respective expression profiles of L. hongkongensis cells grown in modified M63 media supplemented with or without malate using high-throughput RNA-sequencing.

2019-09-30 | GSE135058 | GEO

The Malate Shuttle Detoxifies Ammonia in Exhausted T Cells by Producing 2-Ketoglutarate

Project description:The malate shuttle is traditionally known to maintain the NAD+/NADH balance between the cytosol and mitochondria. Whether the malate shuttle has additional functions was unknown. Here we show that chronic viral infections induced the expression of GOT1, the key enzyme in the malate shuttle, in CD8+ T cells. Got1 deficiency indeed decreased the NAD+/NADH ratio and dampened antiviral CD8+ T cell responses to chronic infection; however, increasing the NAD+/NADH ratio did not restore antiviral T cell responses. Got1 deficiency reduced the production of the ammonia scavenger 2-ketoglutarate and led to toxic ammonia accumulation in CD8+ T cells. Supplementation with 2-ketoglutarate assimilated and detoxified ammonia in Got1-deficient T cells and restored antiviral responses. This study suggests that the major function of the malate shuttle in CD8+ T cells is not to maintain the NAD+/NADH ratio, but rather to detoxify ammonia and enable sustainable ammonia-neutral glutamine catabolism in CD8+ T cells during chronic infections.

2023-09-01 | GSE220876 | GEO

Investigating the RNAs bound by Malate Dehydrgenase 2

Project description:Mitochondrial Malate Dehydrogenase 2 has been identified as an RNA-binding protein in several RNA-interactome capture studies. Here, we used eCLIP to identify the RNA targets bound by human MDH2. We identify cytosolic RNA targets for MDH2, as well as several mitochondrial tRNAs.

2024-06-26 | GSE249145 | GEO

Transcriptomics of pig vascular endothelial cells by D-malate treatment

Project description:By transcriptomics analysis, we identified that the mechanism underlying the effects of D-malate is dependent on the elevated intra-cellular acetyl-CoA content and increased Cyclin A acetylation. This finding revealed a novel mechanism, by which gut microbes impair muscle angiogenesis, and may provide therapeutic target for skeletal muscle function and other vascular associated diseases, such as cancer or aging.

2023-08-02 | GSE239494 | GEO

Investigating the RNAs bound by Malate Dehydrgenase 2

Project description:Investigating the RNAs bound by Malate Dehydrgenase 2

| PRJNA1047543 | ENA

Transcription profiling of Arabidopsis plants after treatment with aluminum

Project description:Aluminum (Al) toxicity, which is caused by the solubilization of Al3 in acid soils resulting in inhibition of root growth and nutrient/water acquisition, is a serious limitation to crop production, because up to one-half of the world?s potentially arable land is acidic. To date, however, no Al tolerance genes have yet been cloned. The physiological mechanisms of tolerance are somewhat better understood; the major documented mechanism involves the Al-activated release of Al-binding organic acids from the root tip, preventing uptake into the primary site of toxicity. In this study, a quantitative trait loci analysis of Al tolerance in Arabidopsis was conducted, which also correlated Al tolerance quantitative trait locus (QTL) with physiological mechanisms of tolerance. The analysis identified two major loci, which explain approximately 40% of the variance in Al tolerance observed among recombinant inbred lines derived from Landsberg erecta (sensitive) and Columbia (tolerant). We characterized the mechanism by which tolerance is achieved, and we found that the two QTL cosegregate with an Al-activated release of malate from Arabidopsis roots. Although only two of the QTL have been identified, malate release explains nearly all (95%) of the variation in Al tolerance in this population. Al tolerance in Landsberg erecta Columbia is more complex genetically than physiologically, in that a number of genes underlie a single physiological mechanism involving root malate release. These findings have set the stage for the subsequent cloning of the genes responsible for the Al tolerance QTL, and a genomics-based cloning strategy and initial progress on this are also discussed.

2007-05-04 | E-SMDB-4017 | biostudies-arrayexpress

Metabolic regulation of the glioblastoma stem cell epitranscriptome by malate dehydrogenase 2 (MDH2)

Project description:Glioblastoma (GBM) ranks among the most lethal of human cancers with current therapies offering only palliation. GBM displays striking intratumoral heterogeneity with diversity of cell state, metabolism, and molecular regulation. Here, we demonstrate that stem-like tumor cells, designated GBM stem cells (GSCs), display higher activity of the malate aspartate shuttle (MAS) through increased expression of malate dehydrogenase 2 (MDH2). Genetic and pharmacologic targeting of MDH2 reduced GSC proliferation, self-renewal, and in vivo tumor growth. MDH2 targeting induced accumulation of alpha-ketoglutarate (αKG), a critical enzymatic co-factor. Mechanistically, MDH2 regulated post-transcriptional regulation of mRNA, specifically N6-Methyladenosine (m6A) with platelet-derived growth factor receptor- (PDGFR) as a regulated transcript. Leveraging these observations, targeting MDH2 and PDGFR displayed combinatorial efficacy against GSCs. Collectively, these results suggest that stem-like tumor cells reprogram their metabolism to induce changes in their epitranscriptomes that reveal possible therapeutic paradigms.

2024-10-15 | GSE255535 | GEO

Aluminum-inducible genes

2007-08-09 | E-GEOD-7582 | biostudies-arrayexpress

2-Stage PAC and syngas into L-malate

Project description:Two-stage conversion of syngas and pyrolysis aqueous condensate into L-malate

| PRJEB72504 | ENA