Single-cell transcriptomic analysis of immune responses within a microfluidic skin equivalent
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ABSTRACT: Microfluidic human skin equivalents (HSEs) were constructed by 3D printing and consisted of an endothelial lined vascular microchannel within a 3D dermal matrix embedded with fibroblasts. Keratinocytes were cultured on the surface to mimic the epidermis. Following exposure of the epidermis to lipopolysaccharide (LPS) and nigericin, human monocytes were injected into the vascular channel and migrated into the HSE. Single-cell RNA sequencing was performed on control or treated HSEs after 1 or 6 days to analyse the inflammatory and immunological responses to LPS and nigericin activation. These studies identified dynamic upregulation and resolution of inflammatory gene signatures in the tissue resident cells in response to treatment, alongside differentiation of monocytes into tissue resident macrophages.
ORGANISM(S): Homo sapiens
PROVIDER: GSE282014 | GEO | 2024/11/22
REPOSITORIES: GEO
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