Dataset Information

Identifying genes directly regulated by Gal1 through its DNA promoter binding in pancreatic stellate cells (PSCs)

ABSTRACT: Galectin-1 (Gal1) is highly expressed in activated PSCs nuclei, a cell type which plays a key role in PDA progression. In order to identify whether Gal-1 is able to recognize regulatory gene elements and regulate their expressión we performed a chromatin immunorecipitation assay followed by sequencing (ChIP-seq). Its analysis revealed that 16% of Gal-1 targets correspond to gene promoters, Indeed, most of these candicates are cancer-associated genes. Among them we found KRAS, which plays an important role in PSC activation. ChIP-seq results were validated by ChIP-qPCR using two independent Gal1 antibodies. These results identify a novel role for nuclear Gal1 by controlling transcriptional gene expression of cancer-associated genes in PSC in the PDA TME.

ORGANISM(S): Homo sapiens

PROVIDER: GSE282020 | GEO | 2025/03/18

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Identification of interactors is a major attempt in cell biology. Not only protein-protein but also protein-carbohydrate interactions are of high relevance for signal transduction in biological systems. Here we aim to identify novel interacting binding partners for the β-galactoside-binding proteins Galectin-1 (Gal-1) and Galectin-3 (Gal-3) in context of the eye disease proliferative vitreoretinopathy (PVR). PVR is one of the most common failures after retinal detachment surgeries and is characterized by the migration, adhesion and epithelial-to-mesenchymal transition (EMT) of retinal pigment epithelial cells (RPE) and the subsequent formation of sub- and epiretinal fibrocellular membranes. Gal-1 and Gal-3 bind in a dose- and carbohydrate-dependent manner to mesenchymal RPE cells and inhibit cellular processes like attachment and spreading. Yet knowledge about glycan-dependent interactors of Gal-1 and Gal-3 on RPE cells is very limited, although this is a prerequisite to unravel the influence of galectins on distinct cellular processes in RPE cells. In this approach, we identified by galectin pull-down experiments and quantitative proteomic screening 131 Galectin-3 and 15 Galectin-1 interactors. They mainly play a role in multiple binding processes and are mostly membrane proteins. Here we focused on two novel identified interactors of Gal-1 and Gal-3 in the context of PVR: the low-density lipoprotein receptor LRP1 and the platelet-derived growth factor receptor beta PDGFRB. We observed crosslinking and lattice formation of exogenous Gal-1 and Gal-3 with LRP1/PDGFRB and ITGB1 on the cell surface of human RPE cells. Weaker binding of Gal-1 and Gal-3 on these interactors and no lattice formation on the cell surface was seen, when complex-type-N-glycosylation was inhibited by treatment of the cells with Kifunensine. In conclusion, the identified specific glycoprotein ligands for Gal-1 and Gal-3 give us new insights in the highly specific binding of galectins to dedifferentiated RPE cells and the resulting prevention of PVR-associated cellular events.

Dataset Information

Identifying genes directly regulated by Gal1 through its DNA promoter binding in pancreatic stellate cells (PSCs)

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets