Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Changes in Gene Expression in Healthcare Workers During Night Shifts: Implications for Immune Response and Health Risks

ABSTRACT: Purpose: This study aimed to analyze gene expression changes in the whole blood of healthcare workers before and after a night shift, to investigate the molecular mechanisms underlying these changes and their potential impact on health. Methods: A single-center, prospective, observational study was conducted involving four medical doctors working night shifts in the emergency department. Blood samples were collected at 10 a.m. on the day of work and at 10 a.m. the following day after the shift. RNA sequencing was performed to analyze gene expression changes, followed by Ingenuity Pathway Analysis (IPA) for core analysis and analysis match. Results: A total of 302 genes were upregulated, and 78 genes were downregulated (p < 0.05, |log2-fold change| > 0.5) after the night shift. Core analysis consistently predicted the activation of immune and inflammatory responses. Analysis match indicated that gene expression patterns after the night shift correlated with several diseases, including major depressive disorder, based on immune and inflammatory response profiles.

ORGANISM(S): Homo sapiens

PROVIDER: GSE282051 | GEO | 2025/03/19

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Human serum proteomics reveal molecular signature after one night of sleep deprivation

Project description:Study Objectives: Sleep deprivation is highly prevalent and caused by conditions such as night shift work or illnesses like obstructive sleep apnea. Compromised sleep is proposed to play a role in several cardiovascular, immune related and neurodegenerative disorders. We recently published human serum proteome changes after a simulated night shift. This study aimed to further explore changes in the human blood serum after 6h of sleep deprivation at night by proteomics and systems biological databases. Methods: Human blood serum samples from 8 self-declared healthy females were analyzed using mass spectrometry and high-pressure liquid chromatography. Each subject was their own control, and two samples were taken from each subject, the first one after 6h of sleep at night and the second one after 6h of sleep deprivation the following night. Biological databases and bioinformatic software were used for systems biological analyzes and comparative analysis with other published sleep-related datasets. Results: Of 494 proteins, 66 were found to be differentially expressed after 6h of sleep deprivation at night. Functional enrichment analysis revealed associations of these proteins with several biological functions related to the regulation of cellular processes like protein- and ion-binding connected to platelet degranulation and blood coagulation, as well as associations with different curated gene sets. Conclusions: This study presents serum proteomic changes after 6h of sleep deprivation, supports previous findings that only 6h of sleep deprivation affects several biological processes and revealed a molecular signature of protein changes related to pathological conditions like altered coagulation and platelet function, impaired lipid and immune function and cancer. Keywords: Human blood serum, proteomics, sleep deprivation, cellular stress, functional enrichment analysis

2024-06-27 | PXD045729 | Pride

The Transcriptomic Regulation of Berry Development is More Important at Night than During the Day

Project description:In order to circumvent environmental changes throughout fruit development, young and ripening berries were sampled simultaneously on continuously flowering microvines acclimated to controlled circadian light and temperature changes. Gene expression profiles along fruit development were monitored during both day and night with whole genome microarray Nimbelgen® vitis 12x, yielding a total number of 9273 developmentally modulated probesets. All day-detected transcripts were modulated at night, whereas 1755 genes were night-specific. Very similar developmental patterns of gene expression were observed upon independent hierarchical clustering of day and night data, whereas functional categories of allocated transcripts varied according to time of the day. Many transcripts within pathways, known to be upregulated during ripening, in particular those linked to secondary metabolism exhibited a clearer developmental regulation at night than during the day. Functional enrichment analysis also indicated that diurnally modulated genes considerably varied during fruit development, with a shift from cellular organization and photosynthesis in green berries to secondary metabolism and stress-related genes in ripening ones. These results reveal critical changes in gene expression during night development that differ from day development which have not been observed in other transcriptomic studies on fruit development so far.

2013-12-20 | GSE52829 | GEO

Changes in Gene Expression in Healthcare Workers During Night Shifts: Implications for Immune Response and Health Risks

Project description:Changes in Gene Expression in Healthcare Workers During Night Shifts: Implications for Immune Response and Health Risks

| PRJNA1186716 | ENA

The Transcriptomic Regulation of Berry Development is More Important at Night than During the Day

2013-12-20 | E-GEOD-52829 | biostudies-arrayexpress

Human blood plasma circadian lipidome misalignment due to night shift simulation

Project description:Blood plasma was collected from a total of 14 subjects participating in a between-groups, in-laboratory study. Healthy volunteers (ages 22-34, BMI average 25.7) were assigned to a 3 day simulated night-shift schedule (6 male, 1 female) or a simulated day-shift (i.e., control, 4 male, 3 female) schedule. For each group, this was followed by a 24-h constant routine protocol, during which blood was drawn at 3-h intervals to measure plasma lipid profiles using LC-MS/MS.

| MSV000086152 | MassIVE

Proteomics of human peripheral blood mononuclear cells under normal circadian cycle and under a simulated night shift

Project description:Proteomic data from human peripheral blood mononuclear cells (PBMCs). Samples taken over a 24 hour constant light and diet following 3 days of normal day shift cycle or a night shift - circadian misaligned schedule where sleep and wake cycles were reversed. Time points (in clock time) 1, 7, 14, 21 hours; 6 volunteers for each condition. Samples were digested with trypsin, then analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS processing pipeline.

2023-10-05 | MSV000093054 | MassIVE

Day-night rhythm in murine retinal pigment epithelium

Project description:Murine RPE transcriptomes were generated for 6 time points during a 24 hour period. Our data demonstrates that genes involved in ATP generation have a high expression at night whereas genes involved in β-oxidation and cholesterol synthesis show a high expression in the morning. Additionally, genes were identified in the glycerophospholipid metabolism pathway, suggesting the generation of glycerophospholipids at night. Finally, genes involved in intracellular trafficking with different expression patterns during the day-night cycle were identified and we identified genes involved in actin cytoskeleton building, remodelling and crosslinking with a high expression in the morning.

2019-12-01 | GSE137005 | GEO

Expression data of Arabidopsis thaliana rosettes in an extended night

Project description:Usually starch is nearly depleted at the end of the night. To induce a gradual depletion of carbon, we have analysed the global response of transcripts during an extension of the night, where carbon becomes severely limiting from about four hours onwards. Keywords: time course

2008-02-14 | GSE10016 | GEO

Transcription profiling of Arabidopsis thaliana rosettes IN an extended night

2008-04-09 | E-GEOD-10016 | biostudies-arrayexpress

Proteomic analysis of the effects of exogenous melatonin on the low night temperature resistance in tomato

Project description:Proteomics was used to analyze the effect of 100μM exogenous melatonin on protein expression of tomato leaves under low night temperature stress.

2022-09-01 | PXD036438 |

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data