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Proteomics of human peripheral blood mononuclear cells under normal circadian cycle and under a simulated night shift

ABSTRACT: Proteomic data from human peripheral blood mononuclear cells (PBMCs). Samples taken over a 24 hour constant light and diet following 3 days of normal day shift cycle or a night shift - circadian misaligned schedule where sleep and wake cycles were reversed. Time points (in clock time) 1, 7, 14, 21 hours; 6 volunteers for each condition. Samples were digested with trypsin, then analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS processing pipeline.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Jason E. McDermott

PROVIDER: MSV000093054 | MassIVE | Thu Oct 05 21:01:00 BST 2023

SECONDARY ACCESSION(S): PXD045923

REPOSITORIES: MassIVE

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Publications

Molecular-Level Dysregulation of Insulin Pathways and Inflammatory Processes in Peripheral Blood Mononuclear Cells by Circadian Misalignment.

McDermott Jason E JE Jacobs Jon M JM Merrill Nathaniel J NJ Mitchell Hugh D HD Arshad Osama A OA McClure Ryan R Teeguarden Justin J Gajula Rajendra P RP Porter Kenneth I KI Satterfield Brieann C BC Lundholm Kirsie R KR Skene Debra J DJ Gaddameedhi Shobhan S Dongen Hans P A Van HPAV

Journal of proteome research 20240415 5

Circadian misalignment due to night work has been associated with an elevated risk for chronic diseases. We investigated the effects of circadian misalignment using shotgun protein profiling of peripheral blood mononuclear cells taken from healthy humans during a constant routine protocol, which was conducted immediately after participants had been subjected to a 3-day simulated night shift schedule or a 3-day simulated day shift schedule. By comparing proteomic profiles between the simulated sh ...[more]

PMID: 38619923

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