Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

DNA methylation epigenome analysis of non-transformed breast cells following lipid exposure.

ABSTRACT: The DNA methylation landscape of MCF-10A breast epithelial cells upon octanoic acid (OA) exposure, in the presence or absence of CBR5884, was analyzed using the Illumina Infinium HumanMethylationEPIC microarray.

ORGANISM(S): Homo sapiens

PROVIDER: GSE283780 | GEO | 2025/01/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Changes in histone modifications measured by CUT&RUN after lipid exposure

Project description:The methylation landscape of MCF-10A breast epithelial cells upon octanoic acid (OA) exposure was analyzed using CUT&RUN for the histone marks H3K27me3 and H3K4me3.

2025-01-01 | GSE283777 | GEO

Gene expression profile at the single cell level of tissue derived breast microstructures after lipid exposure

Project description:The effects of the medium-chain fatty acid octanoic acid (OA) on breast tissue were investigated using single-cell RNA sequencing.

2025-01-01 | GSE284557 | GEO

RNA-seq analysis of MCF-10A human mammary epithelial cells following MYC activation

Project description:We profiled gene expression and splicing changes in MCF-10A human mammary epithelial cells expressing MYC fused to a portion of estrogen receptor (MYC-ER) (Eilers et al., 1989; Littlewood et al., 1995). We performed RNA-seq, in triplicate, on 3D-grown MCF-10A MYC-ER cells at 0, 8, and 24 hours (h) after 4-OHT-induced MYC activation. As a control for 4-OHT-induced effects, 3D-grown parental MCF-10A cells lacking the MYC-ER fusion protein were treated with 4-OHT at the same timepoints.

2022-07-11 | GSE181968 | GEO

Lipid Metabolism (LiMe) genes and the genesis of estrogen receptor negative breast cancer [ATAC-seq]

Project description:MCF-10A breast epithelial cells were exposed to fatty acids to determine if lipids engender changes in gene expression associated with malignant transformation.

2021-12-12 | GSE190573 | GEO

Progestin regulation of gene expression in breast cancer and minimally transformed breast cell lines

Project description:Time course of response to synthetic progestin ORG2058 in T-47D and ZR-75-1 breast cancer cell lines and in two PR positive clones of the MCF-10A cell line: AB9 and AB32. Transcriptional response to synthetic progestin, 10nM ORG2058, was compared between the four cell lines at three treatment times.

2012-04-26 | GSE31128 | GEO

Gene expression signatures associated with PI3Kα-induced senescence

Project description:To investigate the mechanisms of PI3Kα-induced senescence, we performed a gene expression microarray analysis with MCF-10A/H and parental MCF-10A cells.

2018-11-28 | GSE123014 | GEO

Validation of the upgraded EPIC DNA methylation microarray (900K EPIC v2.0) for high-throughput profiling of the human DNA methylome

Project description:Technical and biological validation of the new Illumina Infinium HumanMethylationEPIC version 2.0 microarray. Primary normal and tumor tissues and cancer cell lines from different sources were hybridized with the Illumina Infinium HumanMethylationEPIC version 1.0 and version 2.0 to test the robustness of the new version.

2023-02-28 | GSE222919 | GEO

Impact of FOXA1 over-expression on progestin signalling in transformed normal breast cells

Project description:Genome wide gene expression profiling of response to synthetic progestin ORG2058 in AB32 cells, a PR positive clone of the MCF-10A cell line, was determined after lentiviral transduction with an expression construct encoding human FOXA1. Cells were treated for 6h or 24h with 10nM ORG2058 or vehicle, 48h after transduction with the FOXA1 construct or empty vector control. Gene expression was measured in total RNA my Illumina whole genome gene expression array.

2012-04-26 | GSE31127 | GEO

Discovery of immediate YAP target genes with inducible form of YAP

Project description:Microarray analyses with cells/tissues overexpressing YAP have revealed many transcription targets of YAP (Dong et al, 2007; Zhao et al, 2008). However, as YAP induces transformation of non-cancerous cells, we thought many of known targets of YAP may be indirect consequence of transforming property of YAP. To identify the immediate transcription targets for YAP, we utilized immortalized mammary epithelial MCF-10A cells expressing a tamoxifen inducible, hyperactive (S127/381A) YAP mutant (MCF-10A ERT2-YAP 2SA). MCF-10A ERT2 and MCF-10A ERT2-YAP 2SA are generated. Each cell line was treated with 0.1% of ethanol (solvent) or 1uM of 4-hydroxytamoxifen for 2 or 6 hours. This makes 6 samples per set. The experiments were done in duplicate. The expression data from MCF-10A ERT2 and MCF-10A ERT2-YAP 2SA before tamoxifen treatment can serve as control.

2015-11-23 | E-GEOD-60579 | biostudies-arrayexpress

Homo sapiens

Project description:MCF-10A Exome sequencing

| PRJNA267704 | ENA