Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile at the single cell level of tissue derived breast microstructures after lipid exposure

ABSTRACT: The effects of the medium-chain fatty acid octanoic acid (OA) on breast tissue were investigated using single-cell RNA sequencing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE284557 | GEO | 2025/01/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

DNA methylation epigenome analysis of non-transformed breast cells following lipid exposure.

Project description:The DNA methylation landscape of MCF-10A breast epithelial cells upon octanoic acid (OA) exposure, in the presence or absence of CBR5884, was analyzed using the Illumina Infinium HumanMethylationEPIC microarray.

2025-01-01 | GSE283780 | GEO

Changes in histone modifications measured by CUT&RUN after lipid exposure

Project description:The methylation landscape of MCF-10A breast epithelial cells upon octanoic acid (OA) exposure was analyzed using CUT&RUN for the histone marks H3K27me3 and H3K4me3.

2025-01-01 | GSE283777 | GEO

Medium chain fatty acid (MCFAs) synthesis

Project description:Medium chain fatty acid (MCFAs) synthesis Metagenome

| PRJNA950190 | ENA

Systems level analysis of lipid metabolism in oxygen-induced retinopathy [retina_RNAseq]

Project description:Activation of anaplerosis takes away glutamine from the biosynthetic pathway to the energy-producing TCA cycle. Especially, induction of hyperoxia driven anaplerosis in neurovascular tissues such as the retina during early stages of development could deplete biosynthetic precursors from newly proliferating endothelial cells impeding physiological angiogenesis and leading to vasoobliteration. Using an oxygen-induced retinopathy (OIR) mouse model, we investigated the metabolic differences between OIR-resistant BALB/cByJ and OIR susceptible C57BL/6J strains at system levels to understand the molecular underpinnings that potentially contribute to hyperoxia-induced vascular abnormalities in the neural retina. Our systems level in vivo RNA-seq, proteomic, and lipidomic profiling and ex-vivo explant studies show that the medium-chain fatty acids serves as an alternative source to feed the TCA cycle. Our findings strongly implicate that medium-chain fatty acids could suppress glutamine-fueled anaplerosis and ameliorate hyperoxia-induced vascular abnormalities in conditions such as retinopathy of prematurity.

2024-11-15 | GSE281855 | GEO

Systems level analysis of lipid metabolism in oxygen-induced retinopathy [liver RNA-Seq]

2024-11-15 | GSE281854 | GEO

Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment

Project description:Bacterial vaginosis (BV), a common syndrome characterized by Lactobacillus-deficient vaginal microbiota, is associated with adverse health outcomes. BV often recurs after standard antibiotic therapy in part because antibiotics promote microbiota dominance by Lactobacillus iners instead of Lactobacillus crispatus, which has more beneficial health associations. Strategies to promote L. crispatus and inhibit L. iners are thus needed. We show that oleic acid (OA) and similar long-chain fatty acids simultaneously inhibit L. iners and enhance L. crispatus growth. These phenotypes require OA-inducible genes conserved in L. crispatus and related lactobacilli, including an oleate hydratase (ohyA) and putative fatty acid efflux pump (farE). FarE mediates OA resistance, while OhyA is robustly active in the vaginal microbiota and enhances bacterial fitness by biochemically sequestering OA in a derivative form only ohyA-harboring organisms can exploit. OA promotes L. crispatus dominance more effectively than antibiotics in an in vitro BV model, suggesting a metabolite-based treatment approach.

2024-06-26 | GSE270628 | GEO

Extracellular Vesicle-Mediated Metastasis Suppressors NME1 and NME2 Modify Lipid Metabolism in Fibroblasts

Project description:Our aim was to understand how vesicular NME1 and NME2 released by breast cancer cells influence the tumour microenvironment. As a model, we used human invasive breast carcinoma cells overexpressing NME1 or NME2, and first analysed in detail the presence of both isoforms in EV subtypes by capillaryWestern immunoassay (WES) and immunoelectron microscopy. Data obtained by both methods showed that NME1 was present in medium-sized EVs or microvesicles, whereas NME2 was abundant in both microvesicles and small-sized EVs or exosomes. Next, human skin-derived fibroblasts were treated with NME1 or NME2 containing EVs, and subsequently mRNA expression changes in fibroblasts were examined. RNAseq results showed that the expression of fatty acid and cholesterol metabolism-related genes was decreased significantly in response to NME1 or NME2 containing EV treatment. We found that FASN (fatty acid synthase) and ACSS2 (acyl-coenzyme A synthetase short-chain family member 2), related to fatty acid synthesis and oxidation, were underexpressed in NME1/2-EV-treated fibroblasts. Our data show an emerging link between NME-containing EVs and regulation of tumour metabolism.

2022-08-21 | GSE211556 | GEO

Induction of lipid oxidation gene expression by polyunsaturated fatty acids of marine origin in small intestine of mice

Project description:Dietary polyunsaturated fatty acids (PUFA) act as potent natural hypolipidemics and are linked to many health benefits in humans and in animal models. Mice fed long-term a high fat diet, in which medium-chain alpha linoleic acid (ALA) was partially replaced by long-chain docosahexaenoic (DHA) and eicosapentaenoic (EPA) fatty acids, showed reduced accumulation of body fat and prevention of insulin resistance, besides increased mitochondrial beta-oxidation in white adipose tissue and decreased plasma lipids. ALA, EPA and DHA all belong to PUFA of n-3 series. The intestine is a gatekeeper organ for ingested lipids. To examine the potential contribution of the intestine in the beneficial effects of EPA and DHA, this study assessed gene expression changes using whole genome microarray analysis on small intestinal scrapings. The main biological process affected was lipid metabolism. Fatty acid uptake, peroxisomal and mitochondrial beta-oxidation, and omega-oxidation of fatty acids were all increased. Quantitative real time PCR and intestinal fatty acid oxidation measurements ([14C(U)]-palmitate) confirmed significant gene expression differences in a dose-dependent manner. Furthermore, no major changes in the expression of lipid metabolism genes were observed in colonic scrapings. In conclusion, we show that marine n-3 fatty acids regulate small intestinal gene expression patterns. Since this organ contributes significantly to whole organism energy use, this adaptation of the small intestine may contribute to the complex and observed beneficial physiological effects of these natural compounds under conditions that will normally lead to development of obesity and diabetes.

2009-04-01 | GSE11936 | GEO

Effects of Perioperative Rehabilitation on Blood Tumor-Suppressive MicroRNA Levels and Short- and Long-Term Outcomes in Gastrointestinal Cancer. A Single-Arm Open-Label Study

Project description:Interventions: Patients scheduled for primary resection of gastrointestinal cancers will undergo preoperative rehabilitation for two weeks. During the rehabilitation stay, patients will be on a high-protein diet, medium-chain fatty acid intake, and endurance exercise at 50% to 60% of their maximum heart rate twice a day. Primary outcome(s): Evaluation of changes in blood levels of tumor suppressive miR-133b by perioperative rehabilitation intervention in gastrointestinal cancer Study Design: Single arm Non-randomized

| 2724621 | ecrin-mdr-crc

Differential transport pathways of saturated and unsaturated fatty acid esters in male mouse hepatocytes

Project description:Forty medium and long-chain fatty acids and fatty acid esters in the mouse livers/plasma/VLDL were ethylated and analyzed by UHPLC-MS/MS.

2024-12-18 | MSV000096686 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data