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SnRNA-seq of the hippocampus of Mr1+/+ P301S mice and Mr1-/- P301S mice

ABSTRACT: We performed snRNA-seq using 10xgenomics platform to study the role of MAIT cells in regulating neuroinflammation in mice with neurodegenerative tau pathology. MAIT cells are a type of innate-like T cells that are present in the meninges of mice carrying neurodgenerative human tau mutant transgene. This is the first study to examine single nucleus transcriptome profiles of human tau mice lacking MAIT cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE285822 | GEO | 2025/03/26

REPOSITORIES: GEO

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snRNA-seq of the hippocampus of Mr1+/+ P301S mice and Mr1-/- P301S mice

Project description:snRNA-seq of the hippocampus of Mr1+/+ P301S mice and Mr1-/- P301S mice

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Effect of 4.25% PD fluid treatment on the peritoneum of MR1+/+ C57BL/6J and MR1-/- C57BL/6J mice

Project description:Mr1 knockout (Mr1−/−) mice, in which thymic cells lose the ability to promote the development of mature Mucosal-associated invariant T (MAIT) cells, induced systemic MAIT cell depletion. MAIT cells often exhibit altered functions during fibrosis and inflammatory diseases. Here we used intraperitoneal injections of 4.25% PD fluid (0.1 mL/g) daily for six weeks to mimic peritoneal dialysis (PD) related fibrosis in mice, and investigated whether Mr1 knockout could ameliorated PD-induced changes in peritoneal transcriptome. We analyzed bulk RNA-seq of peritoneums from Mr1+/+ + PBS group (n=3), Mr1+/+ + PD group (n=3), Mr1-/- + PBS group (n=2), and Mr1-/- + PD group (n=2), and demonstrated that Mr1 knockout could ameliorated PD-induced activation in fibrosis, hyperglycolysis, and mTORC1 signalings. In addition, the activation scores of these pathways are positively correlated with each other.

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Single cell RNA-seq of metabolite specific T-cells (MR1-restricted) from adult mouse thymus

Project description:Mucosal-Associated Invariant T cells (MAIT cells) have a unique specificity for the microbial metabolite 5-OP-RU presented by the non-classical presentation molecule MR1. Upon activation, they release cytotoxic mediators and engage an antimicrobial activity. As a subset of T lymphocytes, MAIT development occurs in the thymus where they acquire their effector phenotype under the control of the key transcription factor ZBTB16. This particular maturation process is in contrast with conventional T cells that egress the thymus with a naive phenotype before populating the secondary lymphoid organs, and the molecular events driving the MAIT lineage decision are poorly known. In the present work, we evaluated the transcriptional events and the role of the slam-SAP pathway on the lineage decision of MR1-restricted T cells by single cell RNAseq. MAIT cells undergoing positive selection were FACS-sorted with a MR1:5-OP-RU labeled tetramer, from thymus of wild-type and sapKO mice. Their transcriptomes were captured using a 10x chromium system.

2019-07-01 | E-MTAB-7704 | biostudies-arrayexpress

Cdk5i peptide treatment in aged Tau P301S mice

Project description:To investigate the effects of Cdk5i peptide treatment on gene expression in cortical neurons that isolated from aged Tau P301S mice

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Cdk5i peptide treatment in aged Tau P301S mice

Project description:Cdk5i peptide treatment in aged Tau P301S mice

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CNS cell-type specific gene profiling of aging P301S tau transgenic mice

Project description:RNA sequencing of cortex from young (3mo) and old (10mo) P301S tau transgenic mice

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Nanostring gene expression profiling for markers of Alzheimer's disease in MAPT P301S;cTFEB;HSACre and MAPT P301S male and female mice

Project description:To assess the potential neuroprotective effects of overexpressing skeletal muscle Transcription Factor EB (TFEB) signaling in the context of age-associated neurodegenerative disease pathologies, we derived cTFEB;HSACre transgenic mice in the MAPT P301S tau hyperphosphorylated background and used the Nanostring nCounter® Alzheimer’s Disease panel to gain more precise insights into disease-relevant transcriptional changes in the hippocampus in both male and female mice with both Tau hyperphosphorylation and skeletal muscle TFEB overexpression. We confirmed significantly reduced transcriptional activation of the nCounter AD microglial activation module through signature scoring.

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scRNA-sequencing of mouse meningeal T cells

Project description:The purpose of this experiment is to examine the cellular and molecular differences between MAIT cells and traditional CD4/CD8 T cells in the meninges of 7 month old C57BL/6 mice.

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snRNA-seq of whole hearts from adult BL6 mice

Project description:We used snRNA-seq to investigate an entire adult mammalian heart of BL6 mice. Whole hearts were harvested from 4 male mice (12 weeks) after cervical dislocation. The hearts were pooled and nuclei isolated using the Nuclei PURE Prep isolation kit (Sigma-Aldrich, Darmstadt, Germany) according to the manufacturer’s protocol. Sequencing was conducted by Genewiz (Leipzig, Germany) on the 10xGenomics system. Single nuclei were captured in droplet emulsions and snRNA-seq libraries were constructed as per the 10x Genomics protocol using GemCode Single-Cell 3′ Gel Bead and Library V3 Kit. RNA was controlled for sufficient quality on an Agilent 2100 Bioanalyzer system and quantified using a Qubit Fluorometer.

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