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Characterization of AGO1-/AGO4-associated smRNAs

ABSTRACT: To fully characterize smRNAs associated with AGO1 and AGO4, we developed a two-step protocol to purify AGO/smRNA complexes from flowers, leaves, roots and seedlings with enhanced purity, and sequenced the smRNAs by Illuminaâs technology. We identified some additional miRNAs, collateral miRNAs encoded in known miRNA precursors, phased smRNA clusters and nat-siRNAs. Organ specific sequencing provided digital expression profiles of all obtained smRNAs, especially miRNAs.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE28591 | GEO | 2011/04/19

SECONDARY ACCESSION(S): PRJNA139119

REPOSITORIES: GEO

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Characterization of AGO1-/AGO4-associated smRNAs

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