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MethylC-Seq of ago6-2, ago4-6 and ago4-6 ago6-2 double mutants

ABSTRACT: Using MethylC-Seq to provide single-base resolution of DNA methylation status in ago6-2, ago4-6 and ago4-6 ago6-2 double mutants MethylC-Seq: 3 mutants examined, ago6-2, ago4-6 single mutant and ago4-6 ago6-2 double mutants

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jian-Kang Zhu

PROVIDER: E-GEOD-56388 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Specific but interdependent functions for Arabidopsis AGO4 and AGO6 in RNA-directed DNA methylation.

Duan Cheng-Guo CG Zhang Huiming H Tang Kai K Zhu Xiaohong X Qian Weiqiang W Hou Yueh-Ju YJ Wang Bangshing B Lang Zhaobo Z Zhao Yang Y Wang Xingang X Wang Pengcheng P Zhou Jianping J Liang Gaimei G Liu Na N Wang Chunguo C Zhu Jian-Kang JK

The EMBO journal 20141219 5

Argonaute (AGO) family proteins are conserved key components of small RNA-induced silencing pathways. In the RNA-directed DNA methylation (RdDM) pathway in Arabidopsis, AGO6 is generally considered to be redundant with AGO4. In this report, our comprehensive, genomewide analyses of AGO4- and AGO6-dependent DNA methylation revealed that redundancy is unexpectedly negligible in the genetic interactions between AGO4 and AGO6. Immunofluorescence revealed that AGO4 and AGO6 differ in their subnuclear ...[more]

PMID: 25527293

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