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Small RNA Sequencing in Polytrauma Patients: High vs. Low Troponin T Levels

ABSTRACT: In this study, small RNA sequencing was performed on plasma samples from 10 polytraumatized patients (Injury Severity Score, ISS ≥ 16), who were stratified into two groups based on their initial Troponin T (TnT) levels at the emergency room. The high TnT group (n = 5) included patients with TnT concentrations > 50 pg/mL, while the low TnT group (n = 5) included those with TnT concentrations < 12 pg/mL. The aim of this dataset is to identify differentially expressed miRNAs associated with elevated TnT levels, a marker of cardiac injury in trauma patients. Differential expression analysis revealed several candidate miRNAs that may serve as potential biomarkers for cardiac dysfunction. This dataset highlights the diagnostic potential of miRNAs in post-trauma cardiac injury.

ORGANISM(S): Homo sapiens

PROVIDER: GSE286533 | GEO | 2025/03/04

REPOSITORIES: GEO

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Project description:The etiology of trauma-hemorrhage shock-induced acute lung injury has been difficult to elucidate due, at least in part, to the inability of in vivo studies to separate the non-injurious pulmonary effects of trauma-hemorrhage from the tissue injurious ones. To circumvent this in vivo limitation, we utilized a model of trauma-hemorrhagic shock (T/HS) in which T/HS-lung injury was abrogated by dividing the mesenteric lymph duct. In this way, it was possible to separate the pulmonary injurious response from the non-injurious systemic response to T/HS by comparing the pulmonary molecular response of rats subjected to T/HS which did and did not develop lung injury as well as to non-shocked rats. Utilizing high-density oligonucleotide arrays and treatment group comparisons of whole lung tissue collected at 3 hours after the end of the shock or sham-shock period, 139 of the 8,799 assessed genes were differentially expressed. Experiment Overall Design: Four groups of rats (n=3) were studied in order to identify changes in pulmonary gene expression associated with T/HS, both in the presence and absence of lung injury. These included trauma-sham shock (T/SS) rats which had a laparotomy (trauma) but were not subjected to hemorrhagic shock. These rats had no lung injury and served as controls for rats which were subjected to T/HS (laparotomy plus 90 min of shock) and had lung injury. Differences in gene expression between these two groups would represent both the effects of hemorrhagic shock as well as lung injury. To distinguish the gene response of hemorrhagic shock from the gene response associated with lung injury, gene expression was also compared between T/HS rats (hemorrhage and lung injury) and rats subjected to T/HS plus lymph duct ligation (T/HS-LDL), since the T/HS-LDL rats experienced hemorrhagic shock but had no measurable lung injury. Lastly, to identify hemorrhagic shock- modified genes, the pulmonary gene response of T/HS-LDL (hemorrhage without lung injury) were compared to rats subjected to T/SS plus LDL (no hemorrhage or lung injury). Three hours after the end of the 90 min shock or sham-shock period (i.e. 4.5 hrs after the induction of T/HS), the rats were sacrificed and specimens harvested for genechip analysis and histology.

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Small RNA Sequencing in Polytrauma Patients: High vs. Low Troponin T Levels

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