ABSTRACT: Acanthopanax senticosus is a unique wild resource in Northeast China. Its main active ingredient is polysaccharide, which has prominent immunomodulatory effect. In this study, the purified polysaccharide component of Acanthopanax senticosus leaves (ASPS-A1) with strong immunomodulatory activity was isolated by column chromatography, and its structure and properties were characterized by HPGPC, FT-IR. Results showed that ASPS-A1 is mainly composed of α-1,4-D-GalA, α-1,5-L-Ara, and β-1,4-D-Gal. RT-qPCR experiments and RNA-seq analysis was used to study the immunoregulatory mechanism of ASPS-A1. The results showed that ASPS-A1 could significantly up-regulated the levels of cytokines iNOS, IL-1β, IL-6 and TNF-α activated macrophages through MAPK, NF-κB, and Toll-like receptor signaling pathways. Inhibitory experiments were further confirmed that ASPS-A1 promote the expression of iNOS, TNF-α, and IL-6 via TLR4 receptor, and TNF-α and IL-1β via TLR2 receptor. In order to identify the target of ASPS-A1, molecular docking experiments were conducted. The results demonstrated that ASPS-A1 could bind to both TLR4 and TLR2, forming stable complexes with the cavities on the protein surface through hydrogen bonding and hydrophobic interaction. The docking scores indicated that ASPS-A1 could regulate the immune response through TLR2 and TLR4 signaling pathways, with a particularly strong interaction with TLR4. In summary, this study screened and characterized the most immunoreactive components of Acanthopanax senticosus polysaccharide, disclosed the immunomodulatory mechanism of ASPS-A1, and furnished a research basis for its potential application as a natural immune enhancer.
