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Transcriptomic changes in CRC1 and CRC2 patient-derived CRC 3D models after CRISPRi-based KAT2A knockdown

ABSTRACT: We were interested in transcriptional changes after depletion of histone acetyltransferase KAT2A in human colorectal cancer. Therefore, we utilized patient-derived CRC 3D spheroids CRC1 and CRC2 as models, and analyzed global alterations of gene expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE287741 | GEO | 2025/03/28

REPOSITORIES: GEO

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Transcriptomic changes in HT29 cells and a patient-derived CRC 3D model after CRISPRi-based KAT2A knockdown

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Transcriptomic changes in CRC1 and CRC2 patient-derived CRC 3D models after CRISPRi-based KAT2A knockdown

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Transcriptomic changes in HT29 cells and a patient-derived CRC 3D model after CRISPRi-based KAT2A knockdown

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Divergent Functions of Histone Acetyltransferase Paralogs KAT2A and KAT2B in Keratinocyte Stemness and Differentiation

Project description:The mammalian epidermis undergoes constant renewal replenished by a pool of stem cells and terminal differentiation of their progeny. This is accompanied by changes in gene expression and morphology orchestrated, in part, by epigenetic modifiers. Here, we defined the role of histone acetyltransferase KAT2A in epidermal homeostasis and provided a comparative analysis that revealed key functional divergence with its paralogue, KAT2B. In contrast to KAT2B's reported function in epidermal differentiation, KAT2A supports the undifferentiated state in keratinocytes. RNA-seq analysis of KAT2A- and KAT2B- depleted keratinocytes revealed dysregulated epidermal differentiation. Depletion of KAT2A led to premature expression of epidermal differentiation genes in the absence of inductive signals, whilst loss of KAT2B delayed differentiation. KAT2A acetyltransferase activity was indispensable in regulating epidermal differentiation gene expression. The metazoan-specific N-terminus of KAT2A was also required to support its function in keratinocytes. We further showed that the interplay between KAT2A- and KAT2B- mediated regulation was important for normal cutaneous wound healing in vivo. Overall, these findings reveal a distinct mechanism in which keratinocytes utilize a pair of highly homologous histone acetyltransferases to support divergent functions in self-renewal and differentiation processes.

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ACSS2-coupled KAT2A regulates histone H3 lactylation in gene promoter regions and gene expression

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Kat2a regulates hippocampal memory formation [RNA-seq]

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Project description:We sequenced small RNAs from 12 samples extracted from mouse CA1 tissue to generate the first CA1-specific murine miRNome under normal and Kat2a-loss-of-function conditions. Samples were divded in 4 groups: A: Control (n=6), C: Kat2a cKO naÃ¯ve (n=6)

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Histone acetyltransferase Kat2a stabilises pluripotency with control of transcriptional heterogeneity

Project description:Histone acetyltransferase Kat2a stabilises pluripotency with control of transcriptional heterogeneity

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