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Impact of human anti-O-specific polysaccharide (OSP) antibody on Vibrio cholerae O1 in complex media containing mucin

ABSTRACT: Antibodies targeting the O-specific polysaccharide (OSP) of Vibrio cholerae O1 are a main determinant of protection against cholera. These antibodies can agglutinate bacteria, and can also directly inhibit V. cholerae motility, including at sub-agglutinating conditions. In order to evaluate for possible additional impacts of OSP-specific antibody on V. cholerae, we assessed the transcriptional profile of V. cholerae exposed to an anti-OSP human monoclonal antibody (G1), including in the presence of mucin, the principal component of intestinal mucous. We identified a subset of genes whose expression was significantly altered in the presence of anti-OSP antibody and mucin, including those involved in V. cholerae metabolism, transport, stress response, biofilm formation, motility, and secondary messenger signaling. Our results suggest a broad impact of anti-OSP antibodies on V. cholerae in the presence of mucin and identify several possible mechanisms by which anti-OSP antibodies might protect against cholera.

ORGANISM(S): Vibrio cholerae C6706

PROVIDER: GSE287993 | GEO | 2025/02/10

REPOSITORIES: GEO

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