Dataset Information

Chemoproteomic profiling of host and pathogen enzymes active in cholera

ABSTRACT: Activity-based protein profiling (ABPP) is a chemoproteomic tool for detecting active enzymes in complex biological systems. We used ABPP to identify secreted bacterial and host serine hydrolases that are active in animals infected with the cholera pathogen Vibrio cholerae. Four V. cholerae proteases were consistently active in infected rabbits, and one, VC0157 (renamed IvaP), was also active in human cholera stool. Inactivation of IvaP influenced the activity of other secreted V. cholerae and rabbit enzymes in vivo, while genetic disruption of all four proteases increased the abundance and binding of an intestinal lectin—intelectin—to V. cholerae in infected rabbits. Intelectin also bound to other enteric bacterial pathogens, suggesting it may constitute a previously unrecognized mechanism of bacterial surveillance in the intestine that is inhibited by pathogen-secreted proteases. Our work demonstrates the power of activity-based proteomics to reveal host-pathogen enzymatic dialogue in an animal model of infection.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Vibrio Cholerae O1 Str. Vc91

SUBMITTER: Eranthie Weerapana

LAB HEAD: Eranthie Weerapana

PROVIDER: PXD003000 | Pride | 2016-02-22

REPOSITORIES: Pride

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			Action	DRS
	Supplementary_Dataset_1_WT_Infection_DMSO_A_01.raw	Raw
	Supplementary_Dataset_1_WT_Infection_DMSO_A_02.raw	Raw
	Supplementary_Dataset_1_WT_Infection_DMSO_A_03.raw	Raw
	Supplementary_Dataset_1_WT_Infection_DMSO_A_04.raw	Raw
	Supplementary_Dataset_1_WT_Infection_DMSO_A_05.raw	Raw

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Publications

Chemoproteomic profiling of host and pathogen enzymes active in cholera.

Hatzios Stavroula K SK Abel Sören S Martell Julianne J Hubbard Troy T Sasabe Jumpei J Munera Diana D Clark Lars L Bachovchin Daniel A DA Qadri Firdausi F Ryan Edward T ET Davis Brigid M BM Weerapana Eranthie E Waldor Matthew K MK

Nature chemical biology 20160222 4

Activity-based protein profiling (ABPP) is a chemoproteomic tool for detecting active enzymes in complex biological systems. We used ABPP to identify secreted bacterial and host serine hydrolases that are active in animals infected with the cholera pathogen Vibrio cholerae. Four V. cholerae proteases were consistently active in infected rabbits, and one, VC0157 (renamed IvaP), was also active in human choleric stool. Inactivation of IvaP influenced the activity of other secreted V. cholerae and ...[more]

PMID: 26900865

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