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Expression comparison between SMC4 and conventional cultures

ABSTRACT: The undifferentiated state of pluripotent stem cells depends heavily on the culture conditions. We show that a unique combination of small molecules, SMC4, added to culture conditions converts primed pluripotent stem cells to a more naïve state. By conducting Affymetix analysis we show of majority of lineage markers are repressed in SMC4 culture.

ORGANISM(S): Homo sapiens

PROVIDER: GSE28815 | GEO | 2012/03/16

SECONDARY ACCESSION(S): PRJNA138769

REPOSITORIES: GEO

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Expression comparison between SMC4 and conventional cultures

Project description:The undifferentiated state of pluripotent stem cells depends heavily on the culture conditions. We show that a unique combination of small molecules, SMC4, added to culture conditions converts primed pluripotent stem cells to a more naïve state. By conducting Affymetix analysis we show of majority of lineage markers are repressed in SMC4 culture. Human embryonic stem cells were compared to human induced pluripotent stem cells cultured in either primed or naïve conditions.

2012-03-16 | E-GEOD-28815 | biostudies-arrayexpress

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Project description:Human pluripotent stem cells in culture are often associated with the prime state which represents a more developed state relative to the naïve state which is often associated with the inner cell mass and thought to have the potential to give rise to all cell types. We have developed a small molecule-driven cocktail FMM that maintains human pluripotent stem cells in a state similar to the naive state as defined by several properties including gene expression profile. We used global gene expression to compare and contrast induced pluripotent stem cells -- from various starting points -- cultured in either small molecule medium FMM or conventional medium. Somatic cells were induced to reprogram by various methods and maintained in various culture conditions. Each culture condition was analyzed by various methods including global gene expression.

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Secreted footprint of pluripotency in REBL.PAT IPSCs

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