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A Chemically Defined Feeder-free System for the Establishment and Maintenance of the Human Naive Pluripotent State

ABSTRACT: The distinct states of pluripotency in the pre- and post-implantation embryo can be captured in vitro as naive and primed pluripotent stem cell cultures, respectively. The study and application of the naive state remains hampered, particularly in humans, partially due to current culture protocols relying on extraneous undefined factors such as feeders. Here we performed a small-molecule screen to identify compounds that facilitate chemically defined establishment and maintenance of human feeder-independent naive embryonic (FINE) stem cells. The expression profile in genic and repetitive elements of FINE cells resembles the 8-cell-to-morula stage in vivo, and only differs from feeder-dependent naive cells in genes involved in cell-cell/cell-matrix interactions. FINE cells offer several technical advantages, such as increased amenability to transfection and a longer period of genomic stability, compared with feeder-dependent cells. Thus, FINE cells will serve as an accessible and useful system for scientific and translational applications of naïve pluripotent stem cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE288982 | GEO | 2025/02/07

REPOSITORIES: GEO

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Project description:These are 3 TMT10 samples. The indicated "standard" sub-samples all refer to the same biological sample (obtained as a mixture of all other samples), split and tagged as indicated, to play as a reference for normalization. TMT10 sample1 contains the following sub-samples: -TAG 126: Standard. -TAG 127N: Day 3 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium. -TAG 127C: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium. -TAG 128N: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium. -TAG 128C: Not relevant. -TAG 129N: Not relevant. -TAG 129C: Not relevant. -TAG 130N: Not relevant. -TAG 130C: RSet medium. -TAG 131: Standard. TMT10 sample2 contains the following sub-samples: -TAG 126: Standard -TAG 127N: Day 3 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium. -TAG 127C: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium. -TAG 128N: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium. -TAG 128C: Not relevant. -TAG 129N: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium. -TAG 129C: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium. -TAG 130N: Not relevant. -TAG 130C: RSet medium. -TAG 131: Standard. TMT10 sample3 contains the following sub-samples: -TAG 126: Standard. -TAG 127N: Day 3 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium. -TAG 127C: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium. -TAG 128N: Day 2 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium. -TAG 128C: Day 2 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium. -TAG 129N: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium. -TAG 129C: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium. -TAG 130N: Not relevant. -TAG 130C: RSet medium. -TAG 131: Standard. Detailed culture and processing methods are described in Cesare et al, under submission.

			Action	DRS
		Other
	GSE288982_AllNormMethyl.anno.xlsx	Xlsx
	GSE288982_RAW.tar	Raw
	filelist.txt	Txt

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A Chemically Defined Feeder-free System for the Establishment and Maintenance of the Human Naive Pluripotent State

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