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Microarray analysis of concentration-response transcriptomic changes induced by cannabichromene (CBC) in hepatocytes derived from human induced pluripotent stem cells (iPSCs)

ABSTRACT: Transcriptomic changes measured by microarray in iPSC-derived hepatocytes after exposure to varying concentrations of CBC (0.1-40 μM) for 24 h.

ORGANISM(S): Homo sapiens

PROVIDER: GSE291936 | GEO | 2025/03/21

REPOSITORIES: GEO

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Microarray analysis of concentration-response transcriptomic changes induced by cannabinol (CBN) in hepatocytes derived from human induced pluripotent stem cells (iPSCs)

Project description:Transcriptomic changes measured by microarray in iPSC-derived hepatocytes after exposure to varying concentrations of CBN (0.1-20 μM) for 24 h.

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RNA-seq analysis of concentration-response transcriptomic changes induced by cannabichromene (CBC) in hepatocytes derived from human induced pluripotent stem cells (iPSCs)

Project description:Currently little is known about the safety of nonintoxicating cannabinoids such as cannabichromene (CBC). New approach methodologies (NAMs) based on bioinformatic analysis of high-throughput transcriptomic data are gaining increasing importance in risk assessment and regulatory decision-making of data-poor chemicals. In this study, we conducted a concentration response transcriptomic analysis of CBC in hepatocytes derived from human induced pluripotent stem cells (iPSCs). Biological functions and pathways impacted by CBC exposure were identified, including liver metabolism and cellular oxidative stress. In addition, a transcriptomic point-of-departure (tPoD) value was determined for CBC through benchmark concentration (BMC) analysis of the transcriptomic data. The present study demonstrates the potential utility of transcriptomic BMC analysis as a NAM for hazard assessment of data-poor chemicals, improves our understanding of the possible health effects of CBC, and provides important tPoD data that could contribute to inform human safety assessment of cannabinoid compounds.

2025-03-24 | GSE292355 | GEO

Concentration-response transcriptomic changes induced by silver nanoparticles (AgNPs) in hepatocytes derived from human induced pluripotent stem cells (iPSCs)

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Microarray analysis of concentration-response transcriptomic changes induced by cannabichromene (CBC) in hepatocytes derived from human induced pluripotent stem cells (iPSCs)

Project description:Microarray analysis of concentration-response transcriptomic changes induced by cannabichromene (CBC) in hepatocytes derived from human induced pluripotent stem cells (iPSCs)

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RNA-seq analysis of concentration-response transcriptomic changes induced by cannabichromene (CBC) in hepatocytes derived from human induced pluripotent stem cells (iPSCs)

Project description:RNA-seq analysis of concentration-response transcriptomic changes induced by cannabichromene (CBC) in hepatocytes derived from human induced pluripotent stem cells (iPSCs)

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Mutually Exclusive CBC-Containing Complexes Contribute to RNA Fate.

Project description:The nuclear cap-binding complex (CBC) stimulates processing reactions of capped RNAs, including their splicing, 3'-end formation, degradation, and transport. CBC effects are particular for individual RNA families, but how such selectivity is achieved remains elusive. Here, we analyze three main CBC partners known to impact different RNA species. ARS2 stimulates 3'-end formation/transcription termination of several transcript types, ZC3H18 stimulates degradation of a diverse set of RNAs, and PHAX functions in pre-small nuclear RNA/small nucleolar RNA (pre-snRNA/snoRNA) transport. Surprisingly, these proteins all bind capped RNAs without strong preferences for given transcripts, and their steady-state binding correlates poorly with their function. Despite this, PHAX and ZC3H18 compete for CBC binding and we demonstrate that this competitive binding is functionally relevant. We further show that CBC-containing complexes are short lived in vivo, and we therefore suggest that RNA fate involves the transient formation of mutually exclusive CBC complexes, which may only be consequential at particular checkpoints during RNA biogenesis.

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CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing

Project description:The nuclear cap-binding complex (CBC) stimulates multiple steps in several RNA maturation pathways, but how it functions in humans is incompletely understood. For small capped RNAs like pre-snRNAs, the CBC recruits PHAX. Here, we identify the CBCAP complex, composed of CBC, Ars2 and PHAX, and show that both CBCAP and CBC-Ars2 complexes can be reconstituted from recombinant proteins. Ars2 stimulates PHAX binding to the CBC as well as snRNAs 3'-end processing, hereby coupling maturation with export. In vivo, CBC and Ars2 bind similar capped non-coding and coding RNAs, and stimulate their 3M-bM-^@M-^Y-end processing. Strongest effects are displayed for cap-proximal polyadenylation sites, thereby favoring premature transcription termination. Ars2 functions in part through the mRNA 3M-bM-^@M-^Y-end cleavage factor CLP1, which binds RNA Polymerase II through PCF11. Ars2 is thus a major CBC effector that stimulates functional and cryptic 3'-end processing sites. In total 12 samples; 4 control IP, 2 Flag- Ars2 IP and 2 Flag-CBP20 IP; 2 control FFL siRNA and 2 siRNA Ars2

2013-11-08 | E-GEOD-52131 | biostudies-arrayexpress

CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing

Project description:The nuclear cap-binding complex (CBC) stimulates multiple steps in several RNA maturation pathways, but how it functions in humans is incompletely understood. For small capped RNAs like pre-snRNAs, the CBC recruits PHAX. Here, we identify the CBCAP complex, composed of CBC, Ars2 and PHAX, and show that both CBCAP and CBC-Ars2 complexes can be reconstituted from recombinant proteins. Ars2 stimulates PHAX binding to the CBC as well as snRNAs 3'-end processing, hereby coupling maturation with export. In vivo, CBC and Ars2 bind similar capped non-coding and coding RNAs, and stimulate their 3’-end processing. Strongest effects are displayed for cap-proximal polyadenylation sites, thereby favoring premature transcription termination. Ars2 functions in part through the mRNA 3’-end cleavage factor CLP1, which binds RNA Polymerase II through PCF11. Ars2 is thus a major CBC effector that stimulates functional and cryptic 3'-end processing sites.

2013-11-08 | GSE52131 | GEO

Eccesive autophagic degradation of MYLK3 underlies pathology of sunitinib-induced cardiotoxicity identified by mass spectrum

Project description:The mass spectrometry result of control and sunitinib treated iPSC-CMs at the concentration of 3 μm or 10 μm, p62 served as bait.

2023-08-28 | PXD044902 |

RNA-seq analysis of concentration-response transcriptomic changes induced by cannabinol (CBN) in hepatocytes derived from human induced pluripotent stem cells (iPSCs)

Project description:Currently little is known about the safety of nonintoxicating cannabinoids such as cannabinol (CBN). New approach methodologies (NAMs) based on bioinformatic analysis of high-throughput transcriptomic data are gaining increasing importance in risk assessment and regulatory decision-making of data-poor chemicals. In this study, we conducted a concentration response transcriptomic analysis of CBN in hepatocytes derived from human induced pluripotent stem cells (iPSCs). Biological functions and pathways impacted by CBN exposure were identified, including liver metabolism and cellular oxidative stress. In addition, a transcriptomic point-of-departure (tPoD) value was determined for CBN through benchmark concentration (BMC) analysis of the transcriptomic data. The present study demonstrates the potential utility of transcriptomic BMC analysis as a NAM for hazard assessment of data-poor chemicals, improves our understanding of the possible health effects of CBN, and provides important tPoD data that could contribute to inform human safety assessment of cannabinoid compounds.

2025-03-24 | GSE292356 | GEO

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