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Effects of chronic heat exposure on gene expression in abdominal fat of growing broiler chickens.

ABSTRACT: Exposure to chronic heat stress not only reduces body weight gain in growing broilers, but also enhances fat deposition in the abdomen, subcutaneous, and intramuscular. To elucidate this phenomenon, numerous studies have examined changes in the transcripts, protein, and metabolic levels in the liver, the primary site of de novo fat synthesis in birds. On the other hand, changes in adipose tissue, the direct site of fat deposition, have not been unclear. To elucidate the changes in adipose tissue of chickens heat-exposure, we demonstrated the experiments rearing chickens in a heat environment (32°C) for 14 days, and then compared with the control group that reared in thermoneutral condition (22°C), and a pair-fed groups that reared in thermoneutral condition but feed intake was limited to the same levels as that in the heat-exposed group. Despite the feed intake was equal to that of the pair-fed group, the ratio of abdominal fat to body weight in the heat-exposed group was maintained at the same level as that in the control group. In addition, the heat-exposed group showed a significant decrease in the concentration of free fatty acids in plasma compared with the other 2 groups. Transcriptome analysis of abdominal fat between the heat-exposed and control groups revealed 459 differentially expressed genes were indicated. Notably, in heat-exposed group, the expression of genes related to lipid synthesis increased in adipose tissue compared with control group. Taken together, these results indicate that chronic heat has a lipid-deposition-promoting effect on growing broiler chicken adipose tissue

ORGANISM(S): Gallus gallus

PROVIDER: GSE292022 | GEO | 2025/03/15

REPOSITORIES: GEO

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Project description:Chickens divergently selected for either high abdominal fat content (fat genotype) or low abdominal fat content (lean genotype) at SRA-INRA, France were used to profile abdominal adipose gene expression during juvenile development (1 to 11 weeks of age) and to identify differentially expressed genes associated with genotype and age. The fat line (FL) and lean line (LL) chickens are different in various phenotypic and metabolic measurements, including abdominal fatness, plasma glycemia and T3. The FL and LL chickens represent unique models for characterizing biomedical and agricultural traits. The Del-Mar 14K Chicken Integrated Systems microarrays were used for a transcriptional scan in abdominal adipose during juvenile development using a balanced block hybridization design. Fluorescence intensities were normalized within array (without background subtraction), and between array (aquantile method) in LIMMA package R [Smyth, G. K. (2004) Linear models and empirical Bayes methods for assessing differential expression in microarray experiments. Statistical Applications in Genetics and Molecular Biology, Vol. 3, No. 1, Article 3] producing M-values (log-2 expression ratios) and A-values (average log-2 expression values). Normalized values were analyzed using a two factor ANOVA model. A total of 1,020 differentially expressed functional genes were identified (FDR<0.05). Genes were determined to have a significant effect of age (422), genotype (344), or an age by genotype interaction (254). The differentially expressed genes include metabolic enzymes, acute phase proteins, growth factors, coagulation factors, immune factors and transcription factors involved in various pathways. Several of the functional genes are also identified as positional candidate genes within QTLs in an F2 population established from an intercross between the FL and LL lines. Keywords: Divergently selected chickens, fatness, transcriptional profiling, differentially expressed genes A balanced block design was used for microarray hybridizations, where half of the birds of each genotype and age were labeled with Alexa Flour 647 (red) and the other half with Alexa Flour 555 (green). Four biological replicates were used for each genotype (FL or LL) at six different ages (1, 3, 5, 7, 9 and 11 wk).

Project description:Chickens divergently selected for either high abdominal fat content (fat genotype) or low abdominal fat content (lean genotype) at SRA-INRA, France were used to profile abdominal adipose gene expression during juvenile development (1 to 11 weeks of age) and to identify differentially expressed genes associated with genotype and age. The fat line (FL) and lean line (LL) chickens are different in various phenotypic and metabolic measurements, including abdominal fatness, plasma glycemia and T3. The FL and LL chickens represent unique models for characterizing biomedical and agricultural traits. The Del-Mar 14K Chicken Integrated Systems microarrays were used for a transcriptional scan in abdominal adipose during juvenile development using a balanced block hybridization design. Fluorescence intensities were normalized within array (without background subtraction), and between array (aquantile method) in LIMMA package R [Smyth, G. K. (2004) Linear models and empirical Bayes methods for assessing differential expression in microarray experiments. Statistical Applications in Genetics and Molecular Biology, Vol. 3, No. 1, Article 3] producing M-values (log-2 expression ratios) and A-values (average log-2 expression values). Normalized values were analyzed using a two factor ANOVA model. A total of 3,669 unique differentially expressed functional genes were identified (FDR<0.05). Genes were determined to have a significant effect of age (3,222), genotype (344), or an age by genotype interaction (254). The differentially expressed genes include metabolic enzymes, acute phase proteins, growth factors, coagulation factors, immune factors and transcription factors involved in various pathways. Several of the functional genes are also identified as positional candidate genes within QTLs in an F2 population established from an intercross between the FL and LL lines. Keywords: Divergently selected chickens, fatness, transcriptional profiling, differentially expressed genes

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Effects of chronic heat exposure on gene expression in abdominal fat of growing broiler chickens.

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