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Different Mutants in Desulfovibrio vulgaris Hildenborough

ABSTRACT: Using gene expression changes at mid-log phase data from microarray experiments, we investigated the roles of global transcription regulators in D. vulgaris.

ORGANISM(S): Nitratidesulfovibrio vulgaris str. Hildenborough

PROVIDER: GSE29380 | GEO | 2011/11/01

SECONDARY ACCESSION(S): PRJNA140109

REPOSITORIES: GEO

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Different Mutants in Desulfovibrio vulgaris Hildenborough

Project description:Using gene expression changes at mid-log phase data from microarray experiments, we investigated the roles of global transcription regulators in D. vulgaris. Comparison of cells treated with DVU2097, DVU3111, DVU0379, DVU2547 Mutant to untreated Wildtype cells at times of 0 min

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Effect of nitroprusside on the proteomic expression of Desulfovibrio vulgaris Hildenborough

Project description:In this study, the effect of nitroprusside on protein expression of the model sulfate reducing bacterial isolate (Desulfovibrio vulgaris Hildenborough) was investigated. Three different experiments were done with different exposure time to nitroprusside (30 min, 1 hour, and 3 hours). In each experiment, the culture was grown in quadruplet of serum bottles containing 40 ml complex medium (Postgate medium B) under anaerobic condition at 30oC. At mid-log growth phase, each of the quadruplet culture was divided into two (20 ml each) and nitroprusside (0.25 mM) was added to one of each bottle while the other bottles were used as controls. After 30 min, 1 hour, and 3 hours of exposure to nitroprusside, the culture (1 ml) was pelleted and used for proteomic analysis.

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Effect of glutaraldehyde (Glut) on growth physiology and gene expression of Desulfovibrio vulgaris Hildenborough

Project description:The effect of glutaraldehyde stress on Desulfovibrio vulgaris Hildenborough (DvH) gene expression was determined by comparing the gene expression profiles of DvH with glutaraldehyde added at mid-log phase against DvH grown without glutaraldehdyde. Low concentration glutaraldehyde had little influence over instantaneous growth rate constant and final cell density of the culture. Changes in gene expression caused by exposure to glutaraldehyde were determined using full-genome DvH microarrays. A lot of gene expressions were altered by glutaraldehyde. Keywords: Glutaraldehyde Stress

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