Proteomics

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Effect of nitroprusside on the proteomic expression of Desulfovibrio vulgaris Hildenborough

ABSTRACT: In this study, the effect of nitroprusside on protein expression of the model sulfate reducing bacterial isolate (Desulfovibrio vulgaris Hildenborough) was investigated. Three different experiments were done with different exposure time to nitroprusside (30 min, 1 hour, and 3 hours). In each experiment, the culture was grown in quadruplet of serum bottles containing 40 ml complex medium (Postgate medium B) under anaerobic condition at 30oC. At mid-log growth phase, each of the quadruplet culture was divided into two (20 ml each) and nitroprusside (0.25 mM) was added to one of each bottle while the other bottles were used as controls. After 30 min, 1 hour, and 3 hours of exposure to nitroprusside, the culture (1 ml) was pelleted and used for proteomic analysis.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Desulfovibrio Vulgaris Str. Hildenborough

SUBMITTER: Maryam Ataeian  

LAB HEAD: Gerrit Voordouw

PROVIDER: PXD007623 | Pride | 2018-05-17

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20161207_D_vulgaris_hildenborough_uniprot_proteome_UP000002194_plus_Crap.fasta Fasta
Run12_DVNP_1h_B.msf Msf
Run12_DVNP_1h_B.pep.xml Pepxml
Run12_DVNP_1h_B.raw Raw
Run2_DVNP_1h_A.msf Msf
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Publications

Synergy of Sodium Nitroprusside and Nitrate in Inhibiting the Activity of Sulfate Reducing Bacteria in Oil-Containing Bioreactors.

Fida Tekle T TT   Voordouw Johanna J   Ataeian Maryam M   Kleiner Manuel M   Okpala Gloria G   Mand Jaspreet J   Voordouw Gerrit G  

Frontiers in microbiology 20180516

Sodium nitroprusside (SNP) disrupts microbial biofilms through the release of nitric oxide (NO). The actions of SNP on bacteria have been mostly limited to the genera <i>Pseudomonas</i>, <i>Clostridium</i>, and <i>Bacillus</i>. There are no reports of its biocidal action on sulfate-reducing bacteria (SRB), which couple the reduction of sulfate to sulfide with the oxidation of organic electron donors. Here, we report the inhibition and kill of SRB by low SNP concentrations [0.05 mM (15 ppm)] depe  ...[more]

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