Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

K24-1/EGFP R5 RNA Timecourse

ABSTRACT: The promoter upstream from eryAI (eryAp) was cloned in front of EGFP in pIJ8660. This vector was integrated into the chromosome of K24-1, a clean host version of K41-135 (the overproducer strain obtained from Kosan Biosciences). This strain was then grown in shake flask culture in R5 medium. RNA was harvested up through three days. The 12 h RNA sample was used as the reference sample for all RNA hybridizations. A genomic DNA vs. 12 h control was also done to determine initial relative transcript expression. Groups of assays that are related as part of a time series. Keywords: time_series_design

ORGANISM(S): Streptomyces coelicolor

PROVIDER: GSE2997 | GEO | 2005/07/26

SECONDARY ACCESSION(S): PRJNA104771

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

S. erythraea R5 Fall 2002 RNA Timecourse

Project description:Saccharopolyspora erythraea NRRL2338 (wildtype) and K41-135 (overproducer strain from Kosan Biosciences, KOVP) were grown in shake flask cultures in rich medium R5. RNA samples were harvested for each strain over 5 days. 12 h samples taken for each strain were used as their respective reference ("time zero") samples. The genomic DNA control compares NRRL2338 gDNA to NRRL2338 12 h RNA to show relative gene expression at the start of the timecourse. The 12 h RNA control compares initial gene expression between the wildtype and overproducer strains. Groups of assays that are related as part of a time series. Keywords: time_series_design

2005-07-26 | GSE2996 | GEO

S. fradiae WT and KOS155-3C R5/No Glucose 02/09/03

Project description:RNA timecourse data for Streptomyces fradiae wildtype (ATCC19609) and overproducing strain KOS155-3C(RUS). Strains were grown at 30 C in shake flask cultures in R5 medium with no glucose. RNA samples were harvested over 5 days as tylosin was produced. 12 h RNA samples of each strain were used as the reference sample(green) within their respective timecourses. The WT gDNA vs. 12 h RNA control displays relative gene expression at the beginning of the timecourse. The 12 h RNA control compares initial RNA levels between the WT and overproducer. Groups of assays that are related as part of a time series. Keywords: time_series_design

2005-07-26 | GSE2995 | GEO

S. erythraea R5 Fall 2002 RNA Timecourse

2005-07-26 | E-GEOD-2996 | biostudies-arrayexpress

K24-1/EGFP R5 RNA Timecourse

Project description:The promoter upstream from eryAI (eryAp) was cloned in front of EGFP in pIJ8660. This vector was integrated into the chromosome of K24-1, a clean host version of K41-135 (the overproducer strain obtained from Kosan Biosciences). This strain was then grown in shake flask culture in R5 medium. RNA was harvested up through three days. The 12 h RNA sample was used as the reference sample for all RNA hybridizations. A genomic DNA vs. 12 h control was also done to determine initial relative transcript expression. Groups of assays that are related as part of a time series. Computed

2005-07-26 | E-GEOD-2997 | biostudies-arrayexpress

Transcription profiling of Saccharopolyspora erythraea NRRL2338 (wild type) and K41-135 (overproducer strain from Kosan Biosciences, KOVP) grown in shake flask cultures in rich medium R5, time series

2005-07-21 | E-SMDB-2243 | biostudies-arrayexpress

Transcription profiling of Streptomyces coelicolor with promoter upstream from eryAI (eryAp) was cloned in front of EGFP in pIJ8660, time series

2005-07-21 | E-SMDB-2244 | biostudies-arrayexpress

S. fradiae WT and KOS155-3C R5/No Glucose 02/09/03

2005-07-26 | E-GEOD-2995 | biostudies-arrayexpress

Transcription profiling of Streptomyces fradiae wild type time series (ATCC19609) and overproducing strain KOS155-3C(RUS)

2005-07-21 | E-SMDB-2231 | biostudies-arrayexpress