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Transcriptional network governed by IRF8 and/or PU.1 in germinal center B cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE30359 | GEO | 2011/10/15

SECONDARY ACCESSION(S): PRJNA143635

REPOSITORIES: GEO

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Transcriptional network governed by IRF8 and/or PU.1 in germinal center B cells

Project description:This SuperSeries is composed of the following subset Series: GSE30357: Chip-chip from human diffuse large B cell lymphoma cell lines with IRF8 GSE30358: Mouse B cell lymphoma cell lines:IRF8 knockdown cells vs. Control GSE30519: Chip-chip from mouse diffuse large B cell lymphoma cell lines with IRF8 GSE30520: Chip-chip from mouse diffuse large B cell lymphoma cell lines with PU.1 Refer to individual Series

2011-10-14 | E-GEOD-30359 | biostudies-arrayexpress

Transcriptional regulation of germinal center B and plasma cell fates by dynamical control of IRF4

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OBF1 and Oct factors control the germinal center transcriptional program

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Transcription factors IRF8 and PU.1 are required for follicular B cell development and BCL6-driven germinal center responses.

Project description:The IRF and Ets families of transcription factors regulate the expression of a range of genes involved in immune cell development and function. However, the understanding of the molecular mechanisms of each family member has been limited due to their redundancy and broad effects on multiple lineages of cells. Here, we report that double deletion of floxed Irf8 and Spi1 (encoding PU.1) by Mb1-Cre (designated DKO mice) in the B cell lineage resulted in severe defects in the development of follicular and germinal center (GC) B cells. Class-switch recombination and antibody affinity maturation were also compromised in DKO mice. RNA-seq (sequencing) and ChIP-seq analyses revealed distinct IRF8 and PU.1 target genes in follicular and activated B cells. DKO B cells had diminished expression of target genes vital for maintaining follicular B cell identity and GC development. Moreover, our findings reveal that expression of B-cell lymphoma protein 6 (BCL6), which is critical for development of germinal center B cells, is dependent on IRF8 and PU.1 in vivo, providing a mechanism for the critical role for IRF8 and PU.1 in the development of GC B cells.

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Dynamic changes in E protein activity orchestrate germinal center and plasma cell development

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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IRF8-induced monocyte differentiation

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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EZH2 and BCL6 cooperate to assemble CBX8-BCOR Polycomb complex to repress bivalent promoters, mediate germinal center formation and promote lymphomagenesis

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Distinct Metabolic Requirements Regulate B Cell Activation and Germinal Center Responses

Project description:This SuperSeries is composed of the SubSeries listed below.

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Transcriptional mechanism of IRF8 and PU.1 governs microglial activation in neurodegenerative condition.

Project description:Microglial activation occurs in divergent neuropathological conditions. Such microglial event has the key involvement in the progression of CNS diseases. However, the transcriptional mechanism governing microglial activation remains poorly understood. Here, we investigate the microglial response to traumatic injury-induced neurodegeneration by the 3D fluorescence imaging technique. We show that transcription factors IRF8 and PU.1 are both indispensible for microglial activation, as their specific post-developmental deletion in microglia abolishes the process. Mechanistically, we reveal that IRF8 and PU.1 directly target the gene transcription of each other in a positive feedback to sustain their highly enhanced expression during microglial activation. Moreover, IRF8 and PU.1 dictate the microglial response by cooperatively acting through the composite IRF-ETS motifs that are specifically enriched on microglial activation-related genes. This action of cooperative transcription can be further verified biochemically by the synergetic binding of IRF8 and PU.1 proteins to the composite-motif DNA. Our study has therefore elucidated the central transcriptional mechanism of microglial activation in response to neurodegenerative condition.

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Gene expression in germinal center light zone and dark zone B cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2012-06-14 | GSE38712 | GEO