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Comparison of Aspergillus fumigatus transcriptome grown on selected nitrogen sources

ABSTRACT: Transcriptome of A. fumigatus shifted from ammoniumtartrate to different nitrogen sources and incubated for a defined time was compared. After 16h preculture, the fungus was transferred into fresh medium containing ammonium tartrate, sodium nitrate, proline or bsa as nitrogen source. After 1h, fungus was reisolated, RNA was prepared from fungus, transcriptome was assessed and used for further analysis.

ORGANISM(S): Aspergillus fumigatus Aspergillus fumigatus Af293

PROVIDER: GSE30384 | GEO | 2011/10/30

SECONDARY ACCESSION(S): PRJNA143433

REPOSITORIES: GEO

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Comparison of Aspergillus fumigatus transcriptome grown on selected nitrogen sources

Project description:Transcriptome of A. fumigatus shifted from ammoniumtartrate to different nitrogen sources and incubated for a defined time was compared. After 16h preculture, the fungus was transferred into fresh medium containing ammonium tartrate, sodium nitrate, proline or bsa as nitrogen source. After 1h, fungus was reisolated, RNA was prepared from fungus, transcriptome was assessed and used for further analysis. Ammoniumtartrate, sodiumnitrate, BSA and proline as nitrogen sources, 2 biological replicates for each source. A. fumigatus liquid media shifts were performed according to ref. (Narendja et al.,2002) with minor modifications: 200 ml minimal medium base with 5 mM ammonium tartrate was inoculated with 10^8 conidia freshly harvested. A. fumigatus ATCC 46645 conidia were grown at 37°C and 150 rpm for 16 hours. This pre-culture was then harvested, washed liberally with sterile saline and divided into mycelial masses of equal size on a sterile surface. The portions were then added to 100 ml of minimal medium base without nitrogen source. When a defined carbon source was needed, 1% glucose was added. Flasks were incubated for 20 min at 37°C. Thereafter, one of the following sterile nitrogen sources was added: ammonium tartrate to a final concentration of 5 mM, Sodiumnitrate to a final concentration of 10 mM, 1 g proline suspended in 2 ml minimal medium base, or 0.5 g BSA (Albumin Fraktion V, Roth), suspended in 15 ml minimal medium base. The cultures were incubated for another 60 minutes, harvested by filtering through Miracloth, snap frozen in liquid nitrogen, and ground using a cooled mortar to obtain a fine powder.

2011-10-29 | E-GEOD-30384 | biostudies-arrayexpress

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Project description:A microarray analysis to investigate transcriptional responses in the ectomycorrhizal fungus Paxillus involutus, after providing various sources of nitrogen. Five different sources of nitrogen representing various degrees of complexity were provided as patches in peat microcosms for fungal ingrowth: ammonium phosphate (APO), ammonium sulphate (ASU), glutamine (GLN), bovine serum albumin (BSA), and chitin (CHI). After fungal establishment of patches total RNA was isolated and used for global transcriptional analyses using cDNA microarrays. The entire design involved 9 slides (in the range DW2_01--DW2_13), 14 labelled extracts, and 3 biological replicates (R1-R3). The experiment was designed as a loop combining the 5 diffrent samples and including dye-swaps and biological replication. COMMENT: In our analysis of the raw dataset, 3 channels were removed due to poor signal quality: array 12707648b, Cy5 (635nm) channel (GLN R1 sample); array 12707650c, Cy5 (635nm) channel (APO R1 sample); array 12707651b, Cy5 (635nm) channel (APO R1 sample).

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Effect of Artemisinin on Aspergillus fumigatus gene expression program

Project description:We examined the antifungal activity of artemisinin against Aspergillus fumigatus (A. fumigatus), a pathogenic filamentous fungus responsible for allergic and invasive aspergillosis in humans and analyzed transcript profiles of the fungus on exposure to Artemisinin. A. fumigatus spores were cultured for 48 h and then treated with artemisinin (at MIC50 concentration) or solvent control (DMSO) for 3 h to study its transcriptomic profiles.

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Transcriptome study of A. fumigatus hyphae and human neutrophils

Project description:The infections with the human pathogenic fungus A. fumigatus is an emerging problem. The infections process and the reaction of the immune systeme to the infection is still not complete understood. So we analyzed the response of A. fumigatus to human neutrophils to get insights of these interaction.

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Transcriptome response of Aspergillus fumigatus cultures under different temperatures

Project description:Microarray analysis was used to compare gene expression of Aspergillus fumigatus under two different sporulation temperatures, 17oC and 32oC, with an emphasis on genes encoding known or putative allergens of the fungus. The objective of the study was to investigate whether allergenic potencies of A. fumigatus spores produced under different sporulation temperatures would be influenced by temperature-dependent transcriptional regulation of allergenicity genes.

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Transcriptomics analysis of Aspergillus fumigatus co-cultivated with Pseudomonas aeruginosa

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2018-12-09 | GSE122391 | GEO

Aspergillus fumigatus interaction with Klebsiella pneumoniae in biofilm mode

Project description:This project aimed to characterize inter-species interactions between the A. fumigatus and K. pneumoniae microbial pathogens by analyzing the proteomic profiles of single-pathogen cultures and co-cultures. Biofilms of both A. fumigatus and K. pneumoniae were grown for 24 hours at 37°C in 3 mL of YNBP minimal medium [1.7 g/L yeast nitrogen base (YNB) without amino acids, 5g/L ammonium sulfate, 25 mM phosphate buffer pH 7.0 (KH2PO4 + K2HPO4), 2.5 mM N-acetyl-glucosamine, 0.2% glucose, 0.1% maltose]. Protein pellets obtained from these conditions were further analyzed, revealing metabolic rearrangements and differences in cell cycle regulation due to the interaction. Proteomic profiling, in general, provided new insights into the metabolic reshaping critical for the pathogens to navigate in the course of polymicrobial infection. Additionally, an exploration of the produced compounds uncovered potential biomarker candidates.

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Aspergillus fumigatus transcriptome under normoxia and hypoxia conditions

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2013-03-31 | E-GEOD-30579 | biostudies-arrayexpress

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