Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Regulation of miRNA biogenesis by MCPIP1

ABSTRACT: Effect of MCPIP1 knockdown on miRNA expression profile.

ORGANISM(S): Mus musculus Rattus norvegicus Human gammaherpesvirus 8 JC polyomavirus Betapolyomavirus macacae Human immunodeficiency virus 1 Murid gammaherpesvirus 4 Homo sapiens Human betaherpesvirus 5 Human alphaherpesvirus 1 Betapolyomavirus hominis human gammaherpesvirus 4 Murid betaherpesvirus 1

PROVIDER: GSE31091 | GEO | 2011/11/05

SECONDARY ACCESSION(S): PRJNA144707

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Regulation of miRNA biogenesis by MCPIP1

Project description:Effect of MCPIP1 knockdown on miRNA expression profile. HepG2 cells were transfected with control siRNA or siRNA against MCPIP1, and subjected to miRNA microarray analysis.

2011-11-05 | E-GEOD-31091 | biostudies-arrayexpress

Distinct roles of keratinocyte- and myeloid- MCPIP1 in maintaining skin homeostasis

Project description:The morphology of the skin changes upon depletion of MCPIP1 in cells of myeloid origin as well as in keratinocytes. The thicknesses of epidermal and hypodermal layers are significantly increased in mice with loss of epidermal MCPIP1, whereas loss of myeloid MCPIP1 exerts completely opposite effect. Both types of mice show contrary response to the stimulation with 12-O-tetradecanoylphorbol-13-acetate. Skin morphology and inflammatory phenotype of keratinocyte- and myeloid- double MCPIP1 knockout mice is similar to that of the single keratinocyte knockout of MCPIP1. In all, myeloid and epidermal MCPIP1 play significant but distinct roles in the modulation of skin-related processes.

2024-07-09 | GSE253656 | GEO

MCPIP1 functions as a safeguard of early embryonic development

Project description:Expression of zc3h12a encoding Mcpip1 is tightly controlled within first cell divisions of zebrafish. Overexpression of Mcpip1 leads to the profound transcriptomic changes in the developing embryos and impairs early embryonic development of zebrafish.

2023-07-13 | GSE232220 | GEO

Small RNAseq of MV3 melanoma cells overexpressing MCPIP1 protein

Project description:Sequencing of small RNAs in RNA samples from control cells, cells overexpressing wild-type and mutated form of MCPIP1 ribonuclease

2021-03-01 | GSE164227 | GEO

MCPIP1 controls effects of myeloid cells on skin carcinogenesis and hair growth

Project description:Depletion of Mcpip1 in the cells of myeloid origin modifies the sensitivity to the cutaneous chemical carcinogenesis. It attenuates epidermal proliferation and increases the susceptibility to the formation of melanocytic nevi. Thus, myeloid Mcpip1 is an important modulator in the course of skin carcinogenesis.

2023-12-08 | GSE242128 | GEO

Integrative genomics reveal a role for MCPIP1 in adipogenesis and adipocyte metabolism

Project description:Obesity is considered a serious chronic disease, which is associated with increased risk of developing cardiovascular diseases, non-alcoholic fatty liver disease and type 2 diabetes. Monocyte chemoattractant protein-1-induced protein-1 (MCPIP1), also called Regnase-1, is an RNase that decreases stability of transcripts coding for inflammation-related proteins. In addition, MCPIP1 plays an important role in the regulation of adipogenesis in vitro by reducing the expression of key transcription factors, including C/EBPβ and PPARγ, during adipocyte differentiation. Here we present RNA-Seq and proteomic analysis of 3T3-L1 adipocytes overexpressing wild-type MCPIP1 (WTMCPIP1) and mutant MCPIP1 (D141NMCPIP1) at day 2 and 4 of differentiation, respectively. RNA-Seq analysis followed by confirmatory Q-RT-PCR revealed that elevated MCPIP1 levels in 3T3-L1 adipocytes upregulated transcripts encoding proteins involved in signal transmission and cellular remodeling and downregulated transcripts of factors involved in metabolism. These data are consistent with our LC-MS/MS analysis, which showed that MCPIP1 expressing adipocytes exhibit upregulation of proteins involved in cellular organization and movement and decreased levels of proteins involved in lipid and carbohydrate metabolism. Moreover, MCPIP1 adipocytes are characterized by decreased Glut4 levels and impaired glucose uptake. Overall, our ﬁndings demonstrate that MCPIP1 is an important regulator of adipogenesis and adipocyte metabolism.

2020-01-07 | PXD010148 | Pride

CWC15 is required for miRNA accumulation

Project description:To test if CWC15 is required for miRNA accumulation, an artificial miRNA (amiRCWC15) targeting 5’ UTR (Untranslated region) was used to knockdown the transcript levels of CWC15. Next, to test the effect of CWC15 on miRNA accumulation at global levels, flower inflorescences of four-week old col-0 and amiRCWC15 were collected, and total RNA was extracted by Trizol for Illumina deep sequencing analyses of miRNA profile.

2023-05-01 | GSE228510 | GEO

Regulation of miRNA expression by TGF-b in endothelial cells

Project description:Effect of transforming growth factor-beta (TGF-b) on miRNA expression profile in MS1 endothelial cells.

2016-07-31 | GSE76028 | GEO

miRNA regulation - Proteomic response of mammalian cells to miRNA treatment

Project description:Proteomic response of mammalian cells to miRNA treatment

2017-08-28 | PXD005569 | Pride

Amyotrophic Lateral Sclerosis-associated Mutants of SOD1 Modulate miRNA Biogenesis through Aberrant Interactions with Exportin 5

Project description:Mutations in SOD1 (Superoxide Dismutase 1) gene are associated with amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease. By employing ascorbate peroxidase (APEX)-based proximity labeling, coupled with LC-MS/MS analysis, we uncovered 37 and 28 proteins exhibiting higher abundance in the proximity proteomes of SOD1G85R and SOD1G93A, respectively, than that of the wild-type SOD1. Immunoprecipitation followed by western blot analysis confirmed the preferential binding of one of these proteins, exportin 5 (XPO5), toward the two mutants of SOD1 over its wild-type counterpart. In line with the established function of XPO5 in pre-miRNA transport, we observed diminished nucleocytoplasmic transport of pre-miRNAs in cells with ectopic expression of the two SOD1 mutants over those expressing the wild-type counterpart. On the other hand, RT-qPCR results revealed significant elevations in mature miRNA in cells expressing the two SOD1 mutants, which are attributed to diminished inhibitory effect of XPO5 on Dicer-mediated cleavage of pre-miRNA to mature miRNA. Together, our chemoproteomic approach led to the revelation of a novel mechanism through which ALS-associated mutants of SOD1 perturb miRNA biogenesis, i.e., through aberrant binding toward XPO5.

2024-01-26 | PXD035452 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data