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Molecular Organization of Drosophila Neuroendocrine Cells by DIMMED: Global Profiling of Pan-Neuronal DIMMED Expression Effects

ABSTRACT: To amass candidate DIMM targets in addition to Phm (Park et al., 2008a), we used genome-wide microarray profiling by over-expressing DIMM throughout the embryonic nervous system. We compared profiles from experimental (elav>dimm) and control (elav-GAL4) embryos at 22-26 hr and 28-32 hr after egg laying (AEL). The design was intended to identify transcripts consistently up-regulated shortly after the induction of DIMM; in so doing, we could circumvent the lethality that ensues in late embryonic, and/ or by early larval stages, due to pan-neuronal DIMM expression.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE31113 | GEO | 2011/09/06

SECONDARY ACCESSION(S): PRJNA144683

REPOSITORIES: GEO

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Molecular Organization of Drosophila Neuroendocrine Cells by DIMMED: Global Profiling of Pan-Neuronal DIMMED Expression Effects

Project description:To amass candidate DIMM targets in addition to Phm (Park et al., 2008a), we used genome-wide microarray profiling by over-expressing DIMM throughout the embryonic nervous system. We compared profiles from experimental (elav>dimm) and control (elav-GAL4) embryos at 22-26 hr and 28-32 hr after egg laying (AEL). The design was intended to identify transcripts consistently up-regulated shortly after the induction of DIMM; in so doing, we could circumvent the lethality that ensues in late embryonic, and/ or by early larval stages, due to pan-neuronal DIMM expression. Eight microarrays were used to hybridize cDNAs from experimental dimm::myc-expressing and control flies, sampled at two different embryonic stages. Four microarrays were hybridized with cDNA from the dimm::myc-expressing embryos of genotype w;UAS-dimm2A3/+;elav-GAL4/+; (prefix 'ED'). Another four microarrays were hybridized with cDNAs from the control (maternal) strain: w;;elav-GAL4 (prefix 'E'). Samples ED3 and ED4, E3 and E4 were hybridized with cDNA from stage 14 embryos (24-26hr development at 18C). Samples ED1 and ED2, E1 and E2 were hybridized with cDNA from stage 16 embryos (28-32 hr old embryos at 18C). A pair of GeneChip Drosophila Genome 2.0 arrays (Affymetrix Co., Santa Clara, CA) were tested with each RNA sample (two experimental and two control, for each of two time points). Affymetrix GeneChip Drosophila Genome 2.0 Array

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