Project description:This SuperSeries is composed of the following subset Series: GSE31425: BT549 cells with control or NFIL3 shRNA treatment GSE31426: HEK293 cells with control or NFIL3 shRNA treatment Refer to individual Series
Project description:Transcriptional profiling with samples from BT549 cells that were infected with control or NFIL3 shRNA Two condition experiment: control (scr) shRNA (two replicates) and NFIL3 shRNA (two replicates)
Project description:Transcriptional profiling with samples from HEK293 cells that were infected with control or NFIL3 shRNA Two condition experiment: control (scr) shRNA (six replicates) and NFIL3 shRNA (four replicates)
Project description:We performed genome-wide screening using SW480 cells with Gankyrin knockdown on an Affymetrix gene expression array to identify the transcriptional targets of Gankyrin. Transcriptional profiling with samples from SW480 cells that were infected with control or gankyrin shRNA. Two condition experiment: control shRNA (three replicates) and NFIL3 shRNA (three replicates)
Project description:We investigated the function of the mTORC1 substrates and mRNA translation regulators 4EBP1/2 in the response to glucose deprivation. We uncovered that targeting 4EBP1/2 expression had a direct impact on the survival rates of cells challenged with glucose deprivation. To further define the mechanisms underlying 4EBP1/2 function under glucose deprivation, we conducted a proteomics analysis of HEK293 shRNA control and HEK293 shRNA 4EBP1/2 grown in basal media or under glucose deprivation (1 mM glucose containing media) for 30 hours.
Project description:Comparison of gene expression profile of HEK293 cells stably expressing a shRNA control (SilX-CT) or a shRNA against BAHD1 (SilX-BAHD1) We used microarrays to identify the repertoire of genes differentially expressed upon knockdown of the chromatin repressor BAHD1 in human HEK293 cells.
