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The impact of a consortium of fermented milk strains on the gut microbiome of gnotobiotic mice and monozygotic twins. (RNA-Seq)

ABSTRACT: Understanding how the human gut microbiota and host are impacted by probiotic bacterial strains requires carefully controlled studies in humans, and in mouse models of the gut ecosystem where potentially confounding variables that are difficult to control in humans can be constrained. Therefore, we characterized the fecal microbiomes and metatranscriptomes of adult female monozygotic twin pairs through repeated sampling 4 weeks prior to, 7 weeks during, and 4 weeks following consumption of a commercially-available fermented milk product (FMP) containing a consortium of Bifidobacterium animalis subsp. lactis, two strains of Lactobacillus delbrueckii subsp. bulgaricus, Lactococcus lactis subsp. cremoris, and Streptococcus thermophilus. In addition, gnotobiotic mice harboring a 15-species model human gut microbiota whose genomes contain 58,399 known or predicted protein-coding genes were studied prior to and after gavage with all five sequenced FMP strains.

ORGANISM(S): Streptococcus thermophilus Parabacteroides Bacteroides Collinsella Bifidobacterium animalis subsp. lactis CNCM I-2494 Lactobacillus delbrueckii subsp. bulgaricus Streptococcus thermophilus CNCM I-1630 Lactococcus lactis subsp. lactis CNCM I-1631 Clostridia Ruminococcus human gut metagenome Lactobacillus

PROVIDER: GSE31670 | GEO | 2011/11/01

SECONDARY ACCESSION(S): PRJNA145383

REPOSITORIES: GEO

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The impact of a consortium of fermented milk strains on the gut microbiome of gnotobiotic mice and monozygotic twins. (COPRO-Seq)

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The impact of a consortium of fermented milk strains on the gut microbiome of gnotobiotic mice and monozygotic twins. (COPRO-Seq)

Project description:Understanding how the human gut microbiota and host are impacted by probiotic bacterial strains requires carefully controlled studies in humans, and in mouse models of the gut ecosystem where potentially confounding variables that are difficult to control in humans can be constrained. Therefore, we characterized the fecal microbiomes and metatranscriptomes of adult female monozygotic twin pairs through repeated sampling 4 weeks prior to, 7 weeks during, and 4 weeks following consumption of a commercially-available fermented milk product (FMP) containing a consortium of Bifidobacterium animalis subsp. lactis, two strains of Lactobacillus delbrueckii subsp. bulgaricus, Lactococcus lactis subsp. cremoris, and Streptococcus thermophilus. In addition, gnotobiotic mice harboring a 15-species model human gut microbiota whose genomes contain 58,399 known or predicted protein-coding genes were studied prior to and after gavage with all five sequenced FMP strains. 140 samples total. Evaluation of changes in a model community's structure over time after exposure to a consortium of 5 fermented milk product (FMP) strains.

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