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Transcriptional profiling of ICAM-positive and -negative cells from the mouse olfactory epithelium

ABSTRACT: We used trasncriptional profiling of fluorescent activated cell sorting (FACS) purified ICAM1-positive and negative cells from the olfactory epithelium (OE) of three-week old mice to identify genes enriched in the horizontal basal cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE31972 | GEO | 2011/12/07

SECONDARY ACCESSION(S): PRJNA144979

REPOSITORIES: GEO

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Transcriptional profiling of ICAM-positive and -negative cells from the mouse olfactory epithelium

Project description:We used trasncriptional profiling of fluorescent activated cell sorting (FACS) purified ICAM1-positive and negative cells from the olfactory epithelium (OE) of three-week old mice to identify genes enriched in the horizontal basal cells. The olfactory epithelia from P21-P25 CD1 mice (Charles River Laboratories, Wilmington, MA), were isolated, dissociated, and then purified by FACS using a FITC-conjugated antibody for ICAM1 (CD54). ICAM1-positive and negative cell populations were collected and compared. RNA was purified with TRIzol reagent (Invitrogen, Carlsbad, CA), subjected to two rounds of amplification, labeled, and hybridized to Affymetrix Mouse Genome 430.2 GeneChip microarrays (Affymetrix Inc., Santa Clara, CA, USA) using Affymetrix reagents and protocols (http://www.affymetrix.com). There were three experimental replicates, each consisting of the ICAM1 (+) and ICAM1 (-) FACS purified cell populations from individual litters of mice that were dissected/dissociated/FACS-purified using an ICAM1-FITC conjugated antibody; one microarray was used for each ICAM1 (+) and ICAM1 (-) sample for a total of six microarrays. Microarray data were normalized using GCRMA.

2011-12-07 | E-GEOD-31972 | biostudies-arrayexpress

Mouse Olfactory Epithelium, whole olfactory mucosa, mouse

Project description:The lineage of wild-type horizontal basal cells (HBC) stem cells from the olfactory epithelium were profiled by single-cell RNA-Seq (10X v3 chemistry) to identify differences in aged versus not-aged adult stem cells mRNA expression profiles

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Chip-chip from mouse olfactory epithelium (OE) and liver with antibodies against H3K9me3 and H4K20me3

Project description:The Olfactory Receptor (OR) genes are specifically expressed in the MOE in a monogenic and monoallelic fashion. Only 1 out of 2800 alleles is expressed in each olfactory sensory neuron in mice. ChIP-chip from mouse olfactory epithelium (OE) and liver for H3K9me3 and H4K20me3 revealed that the ORs are highly enriched for these modifications in OE but not in liver. 24 samples were analyzed (20 from OE and 4 from liver) with matching inputs as controls. For the OE samples, there were 2-3 replicates for each array.

2011-05-03 | E-GEOD-24420 | biostudies-arrayexpress

Chip-chip from mouse olfactory epithelium (OE) and liver with antibodies against H3K9me3 and H4K20me3

2011-05-03 | GSE24420 | GEO

Genome-wide screen reveals rhythmic regulation of genes involved in odor processing in the olfactory epithelium

Project description:Odor discrimination behavior displays circadian fluctuations in mice indicating that mammalian olfactory function is under control of the circadian system. This is further supported by the facts that odor discrimination rhythms depend on the presence of clock genes and that olfactory tissues contain autonomous circadian clocks. However, the molecular link between circadian function and olfactory processing is still unknown. In order to elucidate the molecular mechanisms underlying this link, we focused on the olfactory epithelium (OE), the primary target of odors and the site of the initial events in olfactory processing. We asked whether olfactory sensory neurons (OSNs) within the OE possess an autonomous circadian clock and whether olfactory pathways are under circadian control. Employing clock gene-driven bioluminescence reporter assays, immunohistochemistry and a time-dependent microarray-based transcriptome analysis on OE samples, we found robust circadian rhythms of core clock genes and their proteins in OSNs, suggesting that the OE indeed contains an autonomous circadian clock. Furthermore, we identified several OSN-specific components of the olfactory pathway that are under circadian control, including several candidates with putative roles in circadian olfactory processing, such as KIRREL2 -- an established factor involved in short-term OSN activation. The spatiotemporal expression patterns of our candidate proteins suggest that they are involved in short-term anabolic processes to rhythmically prepare the cell for peak performances and to promote circadian function of OSNs. We performed a genome-wide expression study with RNA from OE tissue extracted at 4-h intervals in constant darkness from previously entrained mice. Total RNA of four mice per sampling time was pooled in equal amounts.

2015-11-04 | E-GEOD-69853 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of Mock/SARS-CoV-2 Infected humanized ACE2 Mice Olfactory Epithelium and Olfactory Bulb Transcriptomes

Project description:SARS-CoV-2 infection causes a wide spectrum of clinical manifestations in human, and olfactory dysfunction represents as one of the most predictive and common symptoms in COVID-19 patients. Herein we demonstrate intranasal inoculation of SARS-CoV-2 induces robust viral replication in the olfactory epithelium (OE), not olfactory bulb (OB), resulting in transient olfactory dysfunction in humanized ACE2 mice. To decipher the underlying mechanism of the observed olfactory dysfunction in SARS-CoV-2 infected mice at the molecular level, RNA-Seq analyses of the OE and OB samples from SARS-CoV-2 infected mice were performed in comparison with that from the control animals.

2021-07-12 | GSE173186 | GEO

Genome-wide screen reveals rhythmic regulation of genes involved in odor processing in the olfactory epithelium

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Effect of CCL3 on target ablation-induced macrophage recruitment into the olfactory epithelium

Project description:The aim was to determine the identity of genes regulated at 3 d post-olfactory bulbectomy in the olfactory mucosa of carrier-injected Ccl3-/- mice compared with carrier-injected C57BL/6 mice. Total RNA from the OE was hybridized to Affymetrix microarrays. A number of chemokine-, cytokine-, and growth factor-related genes were significantly regulated in the Ccl3-/- mice, and were restored in CCL3 protein-injected Ccl3-/- mice. The results illustrated that CCL3 played a key role in recruitment of macrophages to the OE and provided insight into the genomic regulation involved in OE remodeling. Keywords: other

2004-11-18 | GSE1680 | GEO

Transcription profiling of mouse olfactory epithelium to determine the identity of genes regulated at 3 d post-olfactory bulbectomy in the olfactory mucosa of carrier-injected Ccl3-/- mice compared with carrier-injected C57BL/6 mice

2007-10-13 | E-GEOD-1680 | biostudies-arrayexpress

Deep sequencing of the murine olfactory receptor neuron transcriptome

Project description:The recent development of next-generation sequencing (NGS) techniques encouraged us to assess the transcriptome of the murine OE. We analyzed RNA from OEs of female and male adult mice and from fluorescence-activated cell sorting (FACS)-sorted olfactory receptor neurons (ORNs) obtained from transgenic OMP-GFP mice

2015-01-21 | GSE53793 | GEO

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