Project description:ChIP-chip assays to determine the occupancy of acetylated histone H3 K56 in wildtype, isw1, chd1, isw1 chd1 and ISW1[K227R] yeast. Two color experiment. ChIP/Input. K56ac data normalized to histone H3 and plotted as mutant over wildtype. Biological replicates=3. Please note that H3 data for YMS123-125 & YMS127 is part of the AcH4 file.
Project description:Gene expression microarray experiments to determine the existence of intragenic (cryptic) transcripts in an isw1 chd1 mutant yeast strain.
Project description:This SuperSeries is composed of the following subset Series: GSE32042: Isw1 and Chd1 maintain chromatin organization during transcription GSE32043: Histone exchange in wildtype, isw1, chd1, isw1 chd1 and ioc4 yeast strains GSE32044: Histone H3 K36me3 in wildtype, isw1 and chd1 yeast strains GSE32045: Isw1 and Chd1 maintain chromatin organization during transcription [AcH4 data] GSE36405: Isw1 and Chd1 maintain chromatin organization during transcription [K56ac data] GSE37158: Isw1 and Chd1 maintain chromatin organization during transcription [isw1 chd1 mutant expression] Refer to individual Series
Project description:Gene expression microarray experiments to determine the existence of intragenic (cryptic) transcripts in an isw1 chd1 mutant yeast strain. Two color experiment. Mutant/WT. Biological replicates=3. Hybridisation of same samples to strand-specific gene expression arrays.
Project description:ChIP-chip assays to determine the localization of 3xFLAG tagged Ioc4, Ioc3 & Isw1 in wildtype and set2 yeast. Two color experiment. ChIP/Input. Biological replicates=3.
Project description:Histone exchange in wildtype, isw1, chd1, isw1 chd1 and ioc4 yeast strains. Two color experiment. Deletion mutant vs. WT cells. Biological replicates=3 per IP per cell type.
Project description:ChIP-chip assays to measure the occupancy of histone H3 K36me3 over the yeast genome in wildtype, isw1 and chd1 yeast strains. ChIPs were done with K36me3 antibody (Ab 9050) in G1 arrested yeast cells.