Expression analysis of WS4303 (wild type) vs WS5041 (mir-58 mutants) total mRNA
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ABSTRACT: To discover new miRNA targets, we generated a C. elegans transgenic line expressing a functional N-terminally Tandem Affinity Purification (TAP) tagged ALG-1 protein (C. elegans strain WS4303). We crossed the TAP::ALG-1 transgene into the mir-58(n4640) mutant background to generate the strain WS5041. For simplicity, we will hereafter term the TAP::ALG-1 transgenic animals as “wild type” and the transgenic WS5041 animals as “mir-58”. In addition to immunopurifying the TAP::ALG-1 and associated RNAs from these strains, we also compared total mRNA levels between these two strains.
ORGANISM(S): Caenorhabditis elegans
PROVIDER: GSE32943 | GEO | 2012/03/01
SECONDARY ACCESSION(S): PRJNA154409
REPOSITORIES: GEO
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