Transcriptomics

Dataset Information

0

Catabolite repression of phosphoenolpyruvate carboxykinase by a zinc finger protein under biotin- and pyruvate carboxylase-deficient conditions in Pichia pastoris


ABSTRACT: We have identified a methanol- and biotin-starvation-inducible zinc finger protein named ROP [repressor of phosphoenolpyruvate carboxykinase (PEPCK)] in the methylotrophic yeast Pichia pastoris. When P.pastoris strain GS115 (wild-type, WT) is cultured in biotin-deficient, glucose ammonium (Bio-) medium, growth is suppressed due to the inhibition of anaplerotic synthesis of oxaloacetate, catalysed by the biotin-dependent enzyme pyruvate carboxylase (PC). Deletion of ROP results in a strain (∆ROP) that can grow under biotin-deficient conditions due to derepression of a biotin- and PC-independent pathway of anaplerotic synthesis of oxaloacetate. Northern analysis as well as microarray expression profiling of RNA isolated from WT and ∆ROP strains cultured in Bio(-) medium indicate that expression of the phosphoenolpyruvate carboxykinase gene (PEPCK) is induced in ∆ROP during biotin- or PC-deficiency even under glucose-abundant conditions. There is an excellent correlation between PEPCK expression and growth of ∆ROP in Bio(-) medium, suggesting that ROP-mediated regulation of PEPCK may have a crucial role in the biotin- and PC-independent growth of the ∆ROP strain. To our knowledge, ROP is the first example, of a zinc finger transcription factor involved in the catabolite repression of PEPCK in yeast cells cultured under biotin- or PC-deficient and glucose-abundant conditions.

ORGANISM(S): Komagataella pastoris

PROVIDER: GSE32948 | GEO | 2011/10/14

SECONDARY ACCESSION(S): PRJNA146463

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2011-10-13 | E-GEOD-32948 | biostudies-arrayexpress
2021-09-09 | PXD022622 | Pride
2020-01-28 | PXD016811 | Pride
2014-10-03 | E-GEOD-61985 | biostudies-arrayexpress
2014-10-03 | GSE61985 | GEO
2023-12-29 | GSE199830 | GEO
2023-12-29 | GSE199829 | GEO
2023-12-29 | GSE199828 | GEO
| PRJNA146463 | ENA
2016-03-06 | GSE44928 | GEO