SRNA scr5239 involved in important feedback loop of S. coelicolor central metabolism
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ABSTRACT: The project is focused on the highly conserved sRNA scr5329 in Streptomyces coelicolor. A proteomics approach revealed that the sRNA regulates several metabolic enzymes, among them the Phosphoenolpyruvate-Carboxykinase (PEPCK), a key enzyme of the central carbon metabolism. The sRNA scr5239 promotes its degradation on the post-transcriptional level. The expression itself is dependent on the global transcriptional regulator DasR, which is N-acetylglucosamine-responsive thereby creating a feedback regulation. By post-transcriptional regulation of PEPCK and likely more targets, scr5239 adds an additional layer to the DasR regulatory network, providing a tool to control the metabolism in dependency to the carbon source.
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Streptomyces Coelicolor Bacteria
TISSUE(S): Cell Culture
SUBMITTER: Elena Ossipova
LAB HEAD: Beatix Suess
PROVIDER: PXD016811 | Pride | 2020-01-28
REPOSITORIES: pride
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